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Applied Microbiology and Biotechnology

, Volume 102, Issue 3, pp 1229–1239 | Cite as

Heterologous production of long-chain rhamnolipids from Burkholderia glumae in Pseudomonas putida—a step forward to tailor-made rhamnolipids

  • Andreas Wittgens
  • Beatrix Santiago-Schuebel
  • Marius Henkel
  • Till Tiso
  • Lars Mathias Blank
  • Rudolf Hausmann
  • Diana Hofmann
  • Susanne Wilhelm
  • Karl-Erich Jaeger
  • Frank Rosenau
Biotechnological products and process engineering

Abstract

Rhamnolipids are biosurfactants consisting of rhamnose (Rha) molecules linked through a β-glycosidic bond to 3-hydroxyfatty acids with various chain lengths, and they have an enormous potential for various industrial applications. The best known native rhamnolipid producer is the human pathogen Pseudomonas aeruginosa, which produces short-chain rhamnolipids mainly consisting of a Rha-Rha-C10-C10 congener. Bacteria from the genus Burkholderia are also able to produce rhamnolipids, which are characterized by their long-chain 3-hydroxyfatty acids with a predominant Rha-Rha-C14-C14 congener. These long-chain rhamnolipids offer different physicochemical properties compared to their counterparts from P. aeruginosa making them very interesting to establish novel potential applications. However, widespread applications of rhamnolipids are still hampered by the pathogenicity of producer strains and—even more important—by the complexity of regulatory networks controlling rhamnolipid production, e.g., the so-called quorum sensing system. To overcome encountered challenges of the wild type, the responsible genes for rhamnolipid biosynthesis in Burkholderia glumae were heterologously expressed in the non-pathogenic Pseudomonas putida KT2440. Our results show that long-chain rhamnolipids from Burkholderia spec. can be produced in P. putida. Surprisingly, the heterologous expression of the genes rhlA and rhlB encoding an acyl- and a rhamnosyltransferase, respectively, resulted in the synthesis of two different mono-rhamnolipid species containing one or two 3-hydroxyfatty acid chains in equal amounts. Furthermore, mixed biosynthetic rhlAB operons with combined genes from different organisms were created to determine whether RhlA or RhlB is responsible to define the fatty acid chain lengths in rhamnolipids.

Keywords

Burkholderia glumae Rhamnolipids Biosurfactant Pseudomonas putida Biosynthesis pathway 

Notes

Acknowledgments

The authors are grateful to the Fachagentur Nachwachsende Rohstoffe e. V. (FNR) and the Deutsche Bundesstiftung Umwelt (DBU) for providing financial support.

Authors’ contributions

AW planned and executed the experiments, created figures, and drafted the manuscript; BSS and DH executed structural analysis of rhamnolipids and critically read the manuscript; MH and TT assisted the plasmid characterization and critically read the manuscript; LMB, RH, SW, and KEJ took part in initiating the research and critically read the manuscript; FR initiated the project, supervised the research, coordinated the study, and critically read the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest..

Supplementary material

253_2017_8702_MOESM1_ESM.pdf (538 kb)
ESM 1 (PDF 537 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Andreas Wittgens
    • 1
    • 2
  • Beatrix Santiago-Schuebel
    • 3
  • Marius Henkel
    • 4
  • Till Tiso
    • 5
  • Lars Mathias Blank
    • 5
  • Rudolf Hausmann
    • 4
  • Diana Hofmann
    • 6
  • Susanne Wilhelm
    • 7
    • 2
  • Karl-Erich Jaeger
    • 2
    • 8
  • Frank Rosenau
    • 1
    • 2
  1. 1.Ulm Center for Peptide Pharmaceuticals (U-PEP)Ulm UniversityUlmGermany
  2. 2.Institute for Molecular Enzyme Technology (IMET)Heinrich-Heine-University Düsseldorf, Forschungszentrum Jülich GmbHJülichGermany
  3. 3.Central Institute for Engineering, Electronics and Analytics, Section Analytics (ZEA-3)Forschungszentrum Jülich GmbHJülichGermany
  4. 4.Institute of Food Science and Biotechnology, Department of Bioprocess Engineering (150k)University of HohenheimStuttgartGermany
  5. 5.Institute of Applied Microbiology (iAMB), Aachen Biology and Biotechnology (ABBt)RWTH Aachen UniversityAachenGermany
  6. 6.Institute of Bio- and Geosciences, IBG-3: AgrosphereForschungszentrum Jülich GmbHJülichGermany
  7. 7.iQu Collegiate-DidacticsHeinrich-Heine-University DüsseldorfDüsseldorfGermany
  8. 8.Institute of Bio- and Geosciences, IBG-1: BiotechnologyForschungszentrum Jülich GmbHJülichGermany

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