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Applied Microbiology and Biotechnology

, Volume 101, Issue 18, pp 6929–6939 | Cite as

Development of a bifunctional xylanase-cellulase chimera with enhanced activity on rice and barley straws using a modular xylanase and an endoglucanase procured from camel rumen metagenome

  • Kamran Khalili Ghadikolaei
  • Kambiz Akbari Noghabi
  • Hossein Shahbani Zahiri
Biotechnologically Relevant Enzymes and Proteins
  • 459 Downloads

Abstract

The camel rumen metagenome is an untapped source of glycoside hydrolases. In this study, novel genes encoding for a modular xylanase (XylC) and a cellulase (CelC) were isolated from a camel rumen metagenome and expressed in Escherichia coli BL21 (DE3). XylC with xylanase (Xyn), CBM, and carbohydrate esterase (CE) domains was characterized as a β-1,4-endoxylanase with remarkable catalytic activity on oat-spelt xylan (K cat = 2919 ± 57 s−1). The implication of XylC’s modular structure in its high catalytic activity was analyzed by truncation and fusion construction with CelC. The resulting fusions including Cel-CBM, Cel-CBM-CE, and Xyn-CBM-Cel showed remarkable enhancement in CMCase activity with K cat values of 742 ± 12, 1289 ± 34.5, and 2799 ± 51 s−1 compared to CelC with a K cat of 422 ± 3.5 s−1. It was also shown that the bifunctional Xyn-CBM-Cel with synergistic xylanase/cellulase activities was more efficient than XylC and CelC in hydrolysis of rice and barley straws.

Keywords

Xylanase Cellulase Fusion protein Metagenome Protein engineering 

Notes

Acknowledgements

This work was supported by National Institute of Genetic Engineering and Biotechnology (NIGEB) (Grant Nos. 518 and 578).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Kamran Khalili Ghadikolaei
    • 1
  • Kambiz Akbari Noghabi
    • 1
  • Hossein Shahbani Zahiri
    • 1
  1. 1.Department of Energy and Environmental BiotechnologyNational Institute of Genetic Engineering and Biotechnology (NIGEB)TehranIran

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