Abstract
Aspergillus niger α-glucosidase (ANG), a member of glycoside hydrolase family 31, catalyzes hydrolysis of α-glucosidic linkages at the non-reducing end. In the presence of high concentrations of maltose, the enzyme also catalyzes the formation of α-(1→6)-glucosyl products by transglucosylation and it is used for production of the industrially useful panose and isomaltooligosaccharides. The initial transglucosylation by wild-type ANG in the presence of 100 mM maltose [Glc(α1–4)Glc] yields both α-(1→6)- and α-(1→4)-glucosidic linkages, the latter constituting ~25% of the total transfer reaction product. The maltotriose [Glc(α1–4)Glc(α1–4)Glc], α-(1→4)-glucosyl product disappears quickly, whereas the α-(1→6)-glucosyl products panose [Glc(α1–6)Glc(α1–4)Glc], isomaltose [Glc(α1–6)Glc], and isomaltotriose [Glc(α1–6)Glc(α1–6)Glc] accumulate. To modify the transglucosylation properties of ANG, residue Asn694, which was predicted to be involved in formation of the plus subsites of ANG, was replaced with Ala, Leu, Phe, and Trp. Except for N694A, the mutations enhanced the initial velocity of the α-(1→4)-transfer reaction to produce maltotriose, which was then degraded at a rate similar to that by wild-type ANG. With increasing reaction time, N694F and N694W mutations led to the accumulation of larger amounts of isomaltose and isomaltotriose than achieved with the wild-type enzyme. In the final stage of the reaction, the major product was panose (N694A and N694L) or isomaltose (N694F and N694W).
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Acknowledgments
We thank the staff of the Instrumental Analysis Division of the Creative Research Institution at Hokkaido University for amino acid analysis. This study was supported in part by the JSPS KAKENHI Grant Number JP26292049 (Atsuo Kimura) and by the JSPS and NRF under the Japan-Korea Basic Science Cooperation Program (Atsuo Kimura).
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Ma, M., Okuyama, M., Sato, M. et al. Effects of mutation of Asn694 in Aspergillus niger α-glucosidase on hydrolysis and transglucosylation. Appl Microbiol Biotechnol 101, 6399–6408 (2017). https://doi.org/10.1007/s00253-017-8402-6
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DOI: https://doi.org/10.1007/s00253-017-8402-6