Carbon and hydrogen isotope fractionation during aerobic biodegradation of quinoline and 3-methylquinoline
- 229 Downloads
Compound-specific isotope analysis has been used extensively to investigate the biodegradation of various organic pollutants. To date, little isotope fractionation information is available for the biodegradation of quinolinic compounds. In this study, we report on the carbon and hydrogen isotope fractionation during quinoline and 3-methylquinoline aerobic microbial degradation by a Comamonas sp. strain Q10. Degradation of quinoline and 3-methylquinoline was accompanied by isotope fractionation. Large hydrogen and small carbon isotope fractionation was observed for quinoline while minor carbon and hydrogen isotope fractionation effects occurred for 3-methylquinoline. Bulk carbon and hydrogen enrichment factors (ε bulk) for quinoline biodegradation were −1.2 ± 0.1 and −38 ± 1‰, respectively, while −0.7 ± 0.1 and −5 ± 1‰ for 3-methylquinoline, respectively. This reveals a potential advantage for employing quinoline as the model compound and hydrogen isotope analysis for assessing aerobic biodegradation of quinolinic compounds. The apparent kinetic isotope effects (AKIEC) values of carbon were 1.008 ± 0.0005 for quinoline and 1.0048 ± 0.0005 for 3-methylquinoline while AKIEH values of hydrogen of 1.264 ± 0.011 for quinoline and 1.0356 ± 0.0103 for 3-methylquinoline were obtained. The combined evaluation of carbon and hydrogen isotope fractionation yields Λ values (Λ = Δδ2H/Δδ13C ≈ εHbulk/εCbulk) of 29 ± 2 for quinoline and 8 ± 2 for 3-methylquinoline. The results indicate that the substrate specificity may have a significant influence on the isotope fractionation for the biodegradation of quinolinic compounds. The substrate-specific isotope enrichment factors would be important for assessing the behavior and fate of quinolinic compounds in the environment.
KeywordsQuinoline 3-Methylquinoline Aerobic biodegradation Isotope fractionation Compound-specific isotope analysis
This work was financially supported by National Natural Science Foundation of China (NSFC- 41271460 and 40901250).
Compliance with ethical standards
Studies with human participants or animals
This article does not contain any studies with human participants or animals performed by any of the authors. Informed consent was obtained from all individual participants included in the study.
Conflict of interest
The authors declare that they have no conflict of interest.
- Bergmann FD, Abu Laban NM, Meyer AH, Elsner M, Meckenstock RU (2011) Dual (C, H) isotope fractionation in anaerobic low molecular weight (poly)aromatic hydrocarbon (PAH) degradation: potential for field studies and mechanistic implications. Environ Sci Technol 45:6947–6953CrossRefPubMedGoogle Scholar
- Liang X, Howlett MR, Nelson JL, Grant G, Dworatzek S, Lacrampe-Couloume G, Zinder SH, Edwards EA, Sherwood Lollar B (2011) Pathway-dependent isotope fractionation during aerobic and anaerobic degradation of monochlorobenzene and 1,2,4-trichlorobenzene. Environ Sci Technol 45:8321–8327Google Scholar
- Peschke B, Lingens F (1991) Microbial metabolism of quinoline and related compounds. XII. Isolation and characterization of the quinoline oxidoreductase from Rhodococcus spec. B1 compared with the quinoline oxidoreductase from Pseudomonas putida 86. Biol Chem Hoppe Seyler 372(12):1081–1088CrossRefPubMedGoogle Scholar
- Philipp B, Hoff M, Germa F, Schink B, Beimborn D, Mersch-Sundermann V (2007) Biochemical interpretation of quantitative structure-activity relationships (QSAR) for biodegradation of N-heterocycles: a complementary approach to predict biodegradability. Environ Sci Technol 41:1390–1398CrossRefPubMedGoogle Scholar
- Tobler NB, Hofstetter TB, Schwarzenbach RP (2008) Carbon and hydrogen isotope fractionation during anaerobic toluene oxidation by Geobacter metallireducens with different Fe(III) phases as terminal electron acceptors. Environ Sci Technol 42:7786–7792Google Scholar