Applied Microbiology and Biotechnology

, Volume 101, Issue 17, pp 6841–6847 | Cite as

Effects of gas condition on acetic acid fermentation by Clostridium thermocellum and Moorella thermoacetica (C. thermoaceticum)

  • Harifara Rabemanolontsoa
  • Dung Van Nguyen
  • Piradee Jusakulvjit
  • Shiro SakaEmail author
Bioenergy and biofuels


Fermentation with acetogens can be affected by cultivation gas phase, but to date, there is not enough evidence on that matter for Clostridium thermocellum and Moorella thermoacetica. In this work, the effects of sparged CO2 as well as sparged and non-sparged N2 on these microorganisms were studied using glucose and cellobiose as substrates. It was revealed that sparged CO2 and non-sparged N2 supported growth and acetic acid production by C. thermocellum and M. thermoacetica, while sparged N2 inhibited both of the microorganisms. Notably, part of the sparged CO2 was fermented by the co-culture system and contributed to an overestimation of the products from the actual substrate as well as an erring material balance. The best condition for the co-culture was concluded to be N2 without sparging. These results demonstrate the importance of cultivation conditions for efficient fermentation by anaerobic clostridia species.


Carbon dioxide (CO2Nitrogen (N2Gas sparging Acetic acid fermentation Clostridium thermocellum Moorella thermoacetica (Clostridium thermoaceticum



This work was supported by the Japan Science and Technology Agency (JST) under the Advanced Low Carbon Technology Research and Development Program (ALCA), for which the authors are extremely grateful.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Harifara Rabemanolontsoa
    • 1
  • Dung Van Nguyen
    • 1
  • Piradee Jusakulvjit
    • 1
  • Shiro Saka
    • 1
    Email author
  1. 1.Department of Socio-Environmental Energy Science, Graduate School of Energy ScienceKyoto UniversityKyotoJapan

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