Applied Microbiology and Biotechnology

, Volume 101, Issue 14, pp 5773–5783 | Cite as

Characterization of an Lrp/AsnC family regulator SCO3361, controlling actinorhodin production and morphological development in Streptomyces coelicolor

  • Jing Liu
  • Jie Li
  • Hong Dong
  • Yunfu Chen
  • Yansheng Wang
  • Hang Wu
  • Changrun Li
  • David T. Weaver
  • Lixin Zhang
  • Buchang Zhang
Applied genetics and molecular biotechnology
First Online:
Received:
Revised:
Accepted:

Abstract

Lrp/AsnC family regulators have been found in many bacteria as crucial regulators controlling diverse cellular processes. By genomic alignment, we found that SCO3361, an Lrp/AsnC family protein from Streptomyces coelicolor, shared the highest similarity to the SACE_Lrp from Saccharopolyspora erythraea. Deletion of SCO3361 led to dramatic reduction in actinorhodin (Act) production and delay in aerial mycelium formation and sporulation on solid media. Dissection of the mechanism underlying the function of SCO3361 in Act production revealed that it altered the transcription of the cluster-situated regulator gene actII-ORF4 by directly binding to its promoter. SCO3361 was an auto-regulator and simultaneously activated the transcription of its adjacent divergently transcribed gene SCO3362. SCO3361 affected aerial hyphae formation and sporulation of S. coelicolor by activating the expression of amfC, whiB, and ssgB. Phenylalanine and cysteine were identified as the effector molecules of SCO3361, with phenylalanine reducing the binding affinity, whereas cysteine increasing it. Moreover, interactional regulation between SCO3361 and SACE_Lrp was discovered for binding to each other’s target gene promoter in this work. Our findings indicate that SCO3361 functions as a pleiotropic regulator controlling secondary metabolism and morphological development in S. coelicolor.

Keywords

Lrp/AsnC family Streptomyces coelicolor SCO3361 Act production Morphological development Interactional regulation 
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Notes

Acknowledgements

This work was supported by the National Program on Key Basic Research Project (973 program, 2013CB734000), the National Natural Science Foundation of China (Grant Nos. 31300081, 31570074, 31600064), the Anhui Provincial Natural Science Foundation (Grant No. 1708085QC49), and the Initial Foundation of Doctoral Scientific Research in Anhui University (01001904, J01001935).

Compliance with ethical standards

Ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Competing interests

The authors declare that they have no competing interests.

Supplementary material

253_2017_8339_MOESM1_ESM.pdf (801 kb)
ESM 1 (PDF 801 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Jing Liu
    • 1
  • Jie Li
    • 1
  • Hong Dong
    • 1
  • Yunfu Chen
    • 1
  • Yansheng Wang
    • 1
  • Hang Wu
    • 1
  • Changrun Li
    • 1
  • David T. Weaver
    • 1
  • Lixin Zhang
    • 1
    • 2
  • Buchang Zhang
    • 1
  1. 1.Institute of Health Sciences, School of Chemistry and Chemical Engineering, School of Life SciencesAnhui UniversityHefeiChina
  2. 2.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina

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