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Applied Microbiology and Biotechnology

, Volume 101, Issue 14, pp 5889–5901 | Cite as

Characterization of an autotrophic bioreactor microbial consortium degrading thiocyanate

  • Mathew Paul Watts
  • Liam Patrick Spurr
  • Han Ming Gan
  • John William Moreau
Environmental biotechnology

Abstract

Thiocyanate (SCN) forms as a by-product of cyanidation during gold ore processing and can be degraded by a variety of microorganisms utilizing it as an energy, nitrogen, sulphur and/or carbon source. In complex consortia inhabiting bioreactor systems, a range of metabolisms are sustained by SCN degradation; however, despite the addition or presence of labile carbon sources in most bioreactor designs to date, autotrophic bacteria have been found to dominate key metabolic functions. In this study, we cultured an autotrophic SCN-degrading consortium directly from gold mine tailings. In a batch-mode bioreactor experiment, this consortium degraded 22 mM SCN, accumulating ammonium (NH4 +) and sulphate (SO4 2−) as the major end products. The consortium consisted of a diverse microbial community comprised of chemolithoautotrophic members, and despite the absence of an added organic carbon substrate, a significant population of heterotrophic bacteria. The role of eukaryotes in bioreactor systems is often poorly understood; however, we found their 18S rRNA genes to be most closely related to sequences from bacterivorous Amoebozoa. Through combined chemical and phylogenetic analyses, we were able to infer roles for key microbial consortium members during SCN biodegradation. This study provides a basis for understanding the behaviour of a SCN degrading bioreactor under autotrophic conditions, an anticipated approach to remediating SCN at contemporary gold mines.

Keywords

Bioremediation Thiocyanate Autotrophic Bioreactor Environmental biotechnology 

Notes

Acknowledgements

Funding for this research was provided by Newmarket Gold Inc. We gratefully acknowledge David Coe, Will Wettenhall, Yan Lim and Megan Parnaby for access to the field site, assistance when collecting the samples and providing access to historic chemical data.

Compliance with ethical standards

Funding

This study was funded by Newmarket Gold Inc.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2017_8313_MOESM1_ESM.pdf (978 kb)
ESM 1 (PDF 977 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Mathew Paul Watts
    • 1
  • Liam Patrick Spurr
    • 1
  • Han Ming Gan
    • 2
    • 3
  • John William Moreau
    • 1
  1. 1.School of Earth SciencesUniversity of MelbourneParkvilleAustralia
  2. 2.School of ScienceMonash University MalaysiaPetaling JayaMalaysia
  3. 3.Genomics Facility, Tropical Medicine and Biology PlatformMonash University MalaysiaPetaling JayaMalaysia

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