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Applied Microbiology and Biotechnology

, Volume 101, Issue 12, pp 5081–5088 | Cite as

Transcriptomic profiling and its implications for the H2 production of a non-methanogen deficient in the frhAGB-encoding hydrogenase

  • Seong Hyuk Lee
  • Min-Sik Kim
  • Yun Jae Kim
  • Tae Wan Kim
  • Sung Gyun KangEmail author
  • Hyun Sook LeeEmail author
Applied microbial and cell physiology

Abstract

The F420-reducing hydrogenase of methanogens functions in methanogenesis by providing reduced coenzyme F420 (F420H2) as an electron donor. In non-methanogens, however, their physiological function has not been identified yet. In this study, we constructed an ΔfrhA mutant, whose frhA gene encoding the hydrogenase α subunit was deleted, in the non-methanogenic Thermococcus onnurineus NA1 as a model organism. There was no significant difference in the formate-dependent growth between the mutant and the wild-type strains. Interestingly, the mutation in the frhA gene affected the expression of genes involved in various cellular functions such as H2 oxidation, chemotactic signal transduction, and carbon monoxide (CO) metabolism. Among these genes, the CO oxidation gene cluster, enabling CO-dependent growth and H2 production, showed a 2.8- to 7.0-fold upregulation by microarray-based whole transcriptome expression profiling. The levels of proteins produced by this gene cluster were also significantly increased not only under the formate condition but also under the CO condition. In a controlled bioreactor, where 100% CO was continuously fed, the ΔfrhA mutant exhibited significant increases in cell growth (2.8-fold) and H2 production (3.4-fold). These findings strongly imply that this hydrogenase is functional in non-methanogens and is related to various cellular metabolic processes through an unidentified mechanism. An understanding of the mechanism by which the frhA gene deletion affected the expression of other genes will provide insights that can be applied to the development of strategies for the enhancement of H2 production using CO as a substrate.

Keywords

frhAGB-encoding hydrogenase Thermococcus onnurineus NA1 Carbon monoxide (CO) H2 production Transcriptome 

Notes

Acknowledgments

We thank Dr. Jeong Ho Jeon and Tae-Jun Yang for the skilled technical assistance.

Compliance with ethical standards

Funding

This study was funded by the KIOST in-house program (PE99514), C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning in the Republic of Korea (2015M3D3A1A01064884), and the Development of Technology for Biohydrogen Production Using the Hyperthermophilic Archaea program of the Ministry of Oceans and Fisheries in the Republic of Korea.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2017_8234_MOESM1_ESM.doc (926 kb)
ESM 1 (DOC 926 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Seong Hyuk Lee
    • 1
  • Min-Sik Kim
    • 1
    • 2
  • Yun Jae Kim
    • 1
    • 3
  • Tae Wan Kim
    • 1
    • 3
  • Sung Gyun Kang
    • 1
    • 3
    Email author
  • Hyun Sook Lee
    • 1
    • 3
    Email author
  1. 1.Korea Institute of Ocean Science and TechnologyAnsanRepublic of Korea
  2. 2.Biomass and Waste Energy LaboratoryKorea Institute of Energy ResearchDaejeonRepublic of Korea
  3. 3.Department of Marine BiotechnologyKorea University of Science and TechnologyDaejeonRepublic of Korea

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