Applied Microbiology and Biotechnology

, Volume 101, Issue 11, pp 4683–4690 | Cite as

The susceptibility of Staphylococcus aureus CIP 65.8 and Pseudomonas aeruginosa ATCC 9721 cells to the bactericidal action of nanostructured Calopteryx haemorrhoidalis damselfly wing surfaces

  • Vi Khanh Truong
  • Nipuni Mahanamanam Geeganagamage
  • Vladimir A. Baulin
  • Jitraporn Vongsvivut
  • Mark J. Tobin
  • Pere Luque
  • Russell J. Crawford
  • Elena P. Ivanova
Applied microbial and cell physiology


Nanostructured insect wing surfaces have been reported to possess the ability to resist bacterial colonization through the mechanical rupture of bacterial cells coming into contact with the surface. In this work, the susceptibility of physiologically young, mature and old Staphylococcus aureus CIP 65.8 and Pseudomonas aeruginosa ATCC 9721 bacterial cells, to the action of the bactericidal nano-pattern of damselfly Calopteryx haemorrhoidalis wing surfaces, was investigated. The results were obtained using several surface characterization techniques including optical profilometry, scanning electron microscopy, synchrotron-sourced Fourier transform infrared microspectroscopy, water contact angle measurements and antibacterial assays. The data indicated that the attachment propensity of physiologically young S. aureus CIP 65.8T and mature P. aeruginosa ATCC 9721 bacterial cells was greater than that of the cells at other stages of growth. Both the S. aureus CIP 65.8T and P. aeruginosa ATCC 9721 cells, grown at the early (1 h) and late stationary phase (24 h), were found to be most susceptible to the action of the wings, with up to 89.7 and 61.3% as well as 97.9 and 97.1% dead cells resulting from contact with the wing surface, respectively.


Black damselfly Calopteryx haemorrhoidalis Nanopillar arrays Bactericidal Physiological growth 



We gratefully acknowledge funding from the Marie Curie Actions research program under the EU FP7 Initial Training Network SNAL 608184. This research was undertaken on the Infrared Microscopectroscopy beamline at the Australian Synchrotron, Victoria, Australia.

Compliance with ethical standards

Human and animal rights and informed consent

This paper does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no competing conflict of interest.

Supplementary material

253_2017_8205_MOESM1_ESM.pdf (862 kb)
ESM 1 (PDF 861 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Vi Khanh Truong
    • 1
  • Nipuni Mahanamanam Geeganagamage
    • 1
  • Vladimir A. Baulin
    • 2
  • Jitraporn Vongsvivut
    • 3
  • Mark J. Tobin
    • 3
  • Pere Luque
    • 4
  • Russell J. Crawford
    • 5
  • Elena P. Ivanova
    • 1
  1. 1.School of Science, Faculty of Science, Engineering and TechnologySwinburne University of TechnologyHawthornAustralia
  2. 2.Departament d’Enginyeria QuimicaUniversitat Rovira i VirgiliTarragonaSpain
  3. 3.Infrared Microspectroscopy Beamline, Australian SynchrotronClaytonAustralia
  4. 4.Museu de les Terres de l’EbreAmpostaSpain
  5. 5.School of Science, College of Science, Engineering and HealthRMIT UniversityMelbourneAustralia

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