Applied Microbiology and Biotechnology

, Volume 101, Issue 3, pp 933–949 | Cite as

Recent progress in Bacillus subtilis spore-surface display: concept, progress, and future

  • He WangEmail author
  • Yunxiang Wang
  • Ruijin Yang


With the increased knowledge on spore structure and advances in biotechnology engineering, the newly developed spore-surface display system confers several inherent advantages over other microbial cell-surface display systems including enhanced stability and high safety. Bacillus subtilis is the most commonly used Bacillus species for spore-surface display. The expression of heterologous antigen or protein on the surface of B. subtilis spores has now been practiced for over a decade with noteworthy success. As an update and supplement to other previous reviews, we comprehensively summarize recent studies in the B. subtilis spore-surface display technique. We focus on its benefits as well as the critical factors affecting its display efficiency and offer suggestions for the future success of this field.


Surface display Spore Bacillus subtilis Factor Display efficiency Application 


Compliance with ethical standards


This work was supported by the project from the Scientific Research & Development Fund of Jiyang College, Zhejiang Agriculture and Forestry University (JY2014RC009, JYMS1409) and Food Science and Engineering the most important discipline of Zhejiang province (JYTsp20142101).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Jiyang CollegeZhejiang Agriculture and Forestry UniversityZhujiChina
  2. 2.School of Food Science and TechnologyJiangnan UniversityWuxiChina

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