Lactobacillus plantarum HAC01 regulates gut microbiota and adipose tissue accumulation in a diet-induced obesity murine model
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The functional features of Lactobacillus plantarum HAC01 (HAC01), isolated from fermented Korean kimchi, were studied with regard to the fat mass, immunometabolic biomarkers and dysbiosis in a diet-induced obesity (DIO) murine model. L. rhamnosus GG (LGG) served as reference strain and a PBS-treated group as control. The administration of L. plantarum HAC01 resulted in reduction of the mesenteric adipose depot, the conjunctive tissue closely associated with the gastrointestinal tract, where lipid oxidative gene expression was upregulated compared to the control group. Metagenome analysis of intestinal microbiota showed that both strains HAC01 and LGG influenced specific bacterial families such as the Lachnospiraceae and Ruminococcaceae rather than the phyla Firmicutes and Bacteroidetes as a whole. The relative abundance of the Lachnospiraceae (phylum Firmicutes) was significantly higher in both LAB-treated groups than in the control. Comparing the impact of the two Lactobacillus strains on microbial composition in the gut also suggests strain-specific effects. The study emphasises the need for deeper studies into functional specificity of a probiotic organism at the strain level. Alleviation of obesity-associated dysbiosis by modulation of the gut microbiota appears to be associated with “indicator” bacterial taxa such as the family Lachnospiraceae. This may provide further insight into mechanisms basic to the mode of probiotic action against obesity and associated dysbiosis.
KeywordsLactobacillus plantarum Lactobacillus rhamnosus GG Mesenteric adipose tissue Diet-induced obesity Dysbiosis Gut microbiota
This research was supported by the Korea Institute of Planning and Evaluation Technology in the Ministry of Food, Agriculture, Forestry and Fisheries (IPET), as part of the research project “Modulation of the microbiome with a concomitant anti-obesity effect by Kimchi originated probiotic feeding” (911053-1). We also gratefully acknowledge support from the Bio- and Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning (2016M3A9A5923160).
Compliance with ethical standards
All applicable international, national and institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.
All animal experiments and protocols were approved by the Committee on the Ethics of Animal Experiments of Handong Global University and were in agreement with the guidelines set forth by the Korean Association for Laboratory Animals.
Conflict of interest
The authors declare that they have no conflict of interest.
WH was funded by Ministry of Food, Agriculture, Forestry and Fisheries (IPET), as part of the research project “Modulation of the microbiome with a concomitant anti-obesity effect by Kimchi originated probiotic feeding” (911053-1).
- Ji YS, Kim HN, Park HJ, Lee JE, Yeo SY, Yang JS, Park SY, Yoon HS, Cho GS, Franz CM, Bomba A, Shin HK, Holzapfel WH (2012) Modulation of the murine microbiome with a concomitant anti-obesity effect by Lactobacillus rhamnosus GG and Lactobacillus sakei NR28. Benef Microbes 3(1):13–22. doi: 10.3920/BM2011.0046 CrossRefPubMedGoogle Scholar
- Kadooka Y, Sato M, Imaizumi K, Ogawa A, Ikuyama K, Akai Y, Okano M, Kagoshima M, Tsuchida T (2010) Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial. Eur J Clin Nutr 64(6):636–643. doi: 10.1038/ejcn.2010.19 CrossRefPubMedGoogle Scholar
- Kimura I, Ozawa K, Inoue D, Imamura T, Kimura K, Maeda T, Terasawa K, Kashihara D, Hirano K, Tani T, Takahashi T, Miyauchi S, Shioi G, Inoue H, Tsujimoto G (2013) The gut microbiota suppresses insulin-mediated fat accumulation via the short-chain fatty acid receptor GPR43. Nat Commun 4:1829. doi: 10.1038/ncomms2852 CrossRefPubMedPubMedCentralGoogle Scholar
- Maghbooli Z, Hossein-nezhad A (2015) Transcriptome and molecular endocrinology aspects of epicardial adipose tissue in cardiovascular diseases: a systematic review and meta-analysis of observational studies. Biomed Res Int 2015:926567 12 pages, http://dx.doi.org/10.1155/2015/926567CrossRefPubMedPubMedCentralGoogle Scholar
- Perez-Cobas AE, Gosalbes MJ, Friedrichs A, Knecht H, Artacho A, Eismann K, Otto W, Rojo D, Bargiela R, von Bergen M, Neulinger SC, Daumer C, Heinsen FA, Latorre A, Barbas C, Seifert J, dos Santos VM, Ott SJ, Ferrer M, Moya A (2013) Gut microbiota disturbance during antibiotic therapy: a multi-omic approach. Gut 62(11):1591–1601. doi: 10.1136/gutjnl-2012-303184 CrossRefPubMedGoogle Scholar
- Wang J, Tang H, Zhang C, Zhao Y, Derrien M, Rocher E, van- Hylckama Vlieg JE, Strissel K, Zhao L, Obin M, Shen J (2015) Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice. ISME J 9(1):1–15. doi: 10.1038/ismej.2014.99 CrossRefPubMedGoogle Scholar
- Zeng J, Li YQ, Zuo XL, Zhen YB, Yang J, Liu CH (2008) Clinical trial: effect of active lactic acid bacteria on mucosal barrier function in patients with diarrhoea-predominant irritable bowel syndrome. Alim Pharmacol Therap 28(8):994–1002Google Scholar