Applied Microbiology and Biotechnology

, Volume 101, Issue 2, pp 843–857 | Cite as

Biotechnological potential of Actinobacteria from Canadian and Azorean volcanic caves

  • Cristina Riquelme
  • Maria de Lurdes Enes Dapkevicius
  • Ana Z. Miller
  • Zachary Charlop-Powers
  • Sean Brady
  • Cohord Mason
  • Naowarat Cheeptham
Environmental biotechnology

Abstract

Caves are regarded as extreme habitats with appropriate conditions for the development of Actinobacteria. In comparison with other habitats, caves have not yet been the target of intensive screening for bioactive secondary metabolites produced by actinomycetes. As a primary screening strategy, we conducted a metagenomic analysis of the diversity and richness of a key gene required for non-ribosomal peptide (NRP) biosynthesis, focusing on cave-derived sediments from two Canadian caves (a lava tube and a limestone cave) to help us predict whether different types of caves may harbor drug-producing actinobacteria. Using degenerate PCR primers targeting adenylation domains (AD), a conserved domain in the core gene in NRP biosynthesis, a number of amplicons were obtained that mapped back to biomedically relevant NRP gene cluster families. This result guided our culture-dependent sampling strategy of actinomycete isolation from the volcanic caves of Canada (British Columbia) and Portugal (Azores) and subsequent characterization of their antibacterial and enzymatic activities. Multiple enzymatic and antimicrobial activities were identified from bacterial of the Arthrobacter and Streptomyces genera demonstrating that actinomycetes from volcanic caves are promising sources of antibacterial, antibiofilm compounds and industrially relevant enzymes.

Keywords

Caves Actinobacteria Metagenomics Antimicrobial activity Enzymatic activity 

Supplementary material

253_2016_7932_MOESM1_ESM.pdf (45 kb)
ESM 1(PDF 45 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Food Science and Health Group (CITA-A), Departamento de Ciências AgráriasUniversidade dos AçoresAngra do HeroísmoPortugal
  2. 2.Instituto de Recursos Naturales y Agrobiología de SevillaConsejo Superior de Investigaciones Científicas (IRNAS-CSIC)SevillaSpain
  3. 3.Laboratory of Genetically Encoded Small MoleculesThe Rockefeller UniversityNew YorkUSA
  4. 4.Department of Biological Sciences, Faculty of ScienceThompson Rivers UniversityKamloopsCanada

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