Development of engineered yeast for biosorption of beer haze-active polyphenols
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Compared to most other alcoholic beverages, the shelf life of beer is much more limited due to its instability in the bottle. That instability is most likely to appear as turbidity (haze), even sedimentation, during storage. The haze in beer is mostly caused by colloidal particles formed by interactions between proteins and polyphenols within the beer. Therefore, beers are usually stabilized by removing at least one of these components. We developed and constructed a Saccharomyces cerevisiae strain with a proline-rich QPF peptide attached to the cell wall, using the C-terminal anchoring domain of α-agglutinin. The QPF peptide served to bind polyphenols during fermentation and, thus, to decrease their concentration. Strains displaying QPF were able to bind about twice as much catechin and epicatechin as a control strain displaying only the anchoring domain. All these experiments were done with model solutions. Depending on the concentration of yeast, uptake of polyphenols was 1.7–2.5 times higher. Similarly, the uptake of proanthocyanidins was increased by about 20 %. Since the modification of yeasts with QPF did not affect their fermentation performance under laboratory conditions, the display of QPF appears to be an approach to increase the stability of beer.
KeywordsSaccharomyces cerevisiae QPF peptide Procyanidin removal Beer colloidal stability
This work was supported by the project TE02000177 “Centre for Innovative Use and Strengthening of Competitiveness of Czech Brewery Raw Materials and Products” of the Technology Agency of the Czech Republic.
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- Asano K, Shinagawa K, Hashimoto N (1982) Characterization of haze-forming proteins of beer and their roles in chill haze formation. J Am Soc Brew Chem 40:147–154Google Scholar
- ASBC Methods of Analysis (1982) ASBC Methods of Analysis, online. Beer Method 5. Real Extract. Approved (1958), rev. (1982). American Society of Brewing Chemists, St. Paul, MN, U.S.A. doi: 10.1094/ASBCMOA-Beer-5
- ASBC Methods of Analysis (2004) ASBC Methods of Analysis, online. Beer Method 4. Alcohol. Approved (1958), rev. (2004). American Society of Brewing Chemists, St. Paul, MN, U.S.A. doi: 10.1094/ASBCMOA-Beer-4
- Bamforth CW (1999) Beer haze. J Am Soc Brew Chem 57:81–90Google Scholar
- Magalhaes PJ, Vieira JS, Goncalves LM, Pacheco JG, Guido LF, Barros AA (2010) Isolation of phenolic compounds from hop extracts using polyvinylpolypyrrolidone: characterization by high-performance liquid chromatography-diode array detection-electrospray tandem mass spectrometry. J Chromatogr A 1217:3258–3268CrossRefPubMedGoogle Scholar
- McMurrough I, Kelly R, Byrne J, O’Brien M (1992) Effect of the removal of sensitive proteins and proanthocyanidins on the colloidal stability of lager beer. J Am Soc Brew Chem 50:67–76Google Scholar
- Sambrook JF, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Laboratory Press, Cold Spring HarborGoogle Scholar
- Siebert KJ, Lynn PY (1997) Mechanisms of beer colloidal stabilization. J Am Soc Brew Chem 55:73–78Google Scholar