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Applied Microbiology and Biotechnology

, Volume 100, Issue 21, pp 9013–9022 | Cite as

Wastewater treatment using microalgae: how realistic a contribution might it be to significant urban wastewater treatment?

  • F. Gabriel AciénEmail author
  • C. Gómez-Serrano
  • M. M. Morales-Amaral
  • J. M. Fernández-Sevilla
  • E. Molina-Grima
Mini-Review

Abstract

Microalgae have been proposed as an option for wastewater treatment since the 1960s, but still, this technology has not been expanded to an industrial scale. In this paper, the major factors limiting the performance of these systems are analysed. The composition of the wastewater is highly relevant, and especially the presence of pollutants such as heavy metals and emerging compounds. Biological and engineering aspects are also critical and have to be improved to at least approximate the performance of conventional systems, not just in terms of capacity and efficiency but also in terms of robustness. Finally, the harvesting of the biomass and its processing into valuable products pose a challenge; yet at the same time, an opportunity exists to increase economic profitability. Land requirement is a major bottleneck that can be ameliorated by improving the system’s photosynthetic efficiency. Land requirement has a significant impact on the economic balance, but the profits from the biomass produced can enhance these systems’ reliability, especially in small cities.

Keywords

Microalgae Wastewater Nutrient recovery Photosynthetic efficiency Photobioreactors 

Notes

Acknowledgments

This research was supported by EDARSOL CTQ2014-57293-C3 (Spanish Ministry of Science and Innovation) and the PURALGA RTA2013-0056-C03 (INIA) projects. We are most grateful to the Estación Experimental Las Palmerillas of the Fundación Cajamar for collaborating in this research. This research was supported by the Junta de Andalucía and the Plan Andaluz de Investigación (BIO 173).

Compliance with ethical standards

This paper has been developed accomplishing the guidelines for ethical responsibility of authors. This is an original work performed by the authors that has not been previously submitted to other journal. Data has been not manipulated or altered. The text was written avoiding plagiarism from other previous published papers.

This work is part of the research performed by the authors in several projects, all of them supported by governmental founds, without conflict with companies or other funding entities.

Conflict of interest

Authors declare that they have not conflict of interest on the publication of this paper and data on it contained.

Human and animal rights and informed consent

In this work, no animals or humans are used. This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Acién FG, Fernández Sevilla JM, Molina Grima E (2013) Photobioreactors for the production of microalgae. Rev Environ Sci Biotechnol 12:131–151CrossRefGoogle Scholar
  2. Borde X, Guieysse B, Delgado O, Muñoz R, Hatti-Kaul R, Nugier-Chauvin C, Patin H, Mattiasson B (2003) Synergistic relationships in algal-bacterial microcosms for the treatment of aromatic pollutants. Bioresour Technol 86:293–300CrossRefPubMedGoogle Scholar
  3. Broekhuizen N, Park JBK, McBride GB, Craggs RJ (2012) Modification, calibration and verification of the IWA River water quality model to simulate a pilot-scale high rate algal pond. Water Res 46:2911–2926CrossRefPubMedGoogle Scholar
  4. Carvalho AP, Meireles LA, Malcata FX (2006) Microalgal reactors: a review of enclosed system designs and performances. Biotechnol Prog 22:1490–1506CrossRefPubMedGoogle Scholar
  5. Christenson L, Sims R (2011) Production and harvesting of microalgae for wastewater treatment, biofuels, and bioproducts. Biotechnol Adv 29:686–702CrossRefPubMedGoogle Scholar
  6. Collos Y, Harrison PJ (2014) Acclimation and toxicity of high ammonium concentrations to unicellular algae. Mar Pollut Bull 80:8–23CrossRefPubMedGoogle Scholar
  7. de Godos I, Mendoza JL, Acién FG, Molina E, Banks C, Heaven S, Rogalla F (2014) Evaluation of carbon dioxide mass transfer in raceway reactors for microalgae culture using flue gases. Bioresour Technol 153:307–314CrossRefPubMedGoogle Scholar
  8. Doucha J, Lívanský K (1995) Novel outdoor thin-layer high density microalgal culture system: productivity and operation parameters. Arch Hydrobiol. Algol Stud 76:129–147Google Scholar
  9. Doucha J, Lívanský K (2006) Productivity, CO2/O2 exchange and hydraulics in outdoor open high density microalgal (Chlorella sp.) photobioreactors operated in a middle and southern European climate. J Appl Phycol 18:811–826CrossRefGoogle Scholar
  10. Duarte-Santos T, Mendoza-Martín JL, Acién FG, Molina E, Vieira-Costa JA, Heaven S (2016) Optimization of carbon dioxide supply in raceway reactors: influence of carbon dioxide molar fraction and gas flow rate. Bioresour Technol 212:72–81CrossRefPubMedGoogle Scholar
  11. Ferrero EM, de Godos I, Rodríguez EM, García-Encina PA, Muñoz R, Bácares E (2012) Molecular characterization of bacterial communities in algal-bacterial photobioreactors treating piggery wastewaters. Ecol Eng 40:121–130CrossRefGoogle Scholar
  12. Fouilland E, Vasseur C, Leboulanger C, Le Floc’h E, Carré C, Marty B, Steyer JP, Sialve B (2014) Coupling algal biomass production and anaerobic digestion: production assessment of some native temperate and tropical microalgae. Biomass Bioenergy 70:564–569CrossRefGoogle Scholar
  13. Granados MR, Acién FG, Gómez C, Fernández-Sevilla JM, Molina Grima E (2012) Evaluation of flocculants for the recovery of freshwater microalgae. Bioresour Technol 118:102–110CrossRefPubMedGoogle Scholar
  14. Heaven S, Milledge J, Zhang Y (2011) Comments on “anaerobic digestion of microalgae as a necessary step to make microalgal biodiesel sustainable. Biotechnol Adv 29:164–167CrossRefPubMedGoogle Scholar
  15. Karya NGAI, van der Steen NP, Lens PNL (2013) Photo-oxygenation to support nitrification in an algal-bacterial consortium treating artificial wastewater. Bioresour Technol 134:244–250CrossRefPubMedGoogle Scholar
  16. Lau KY, Pleissner D, Lin CSK (2014) Recycling of food waste as nutrients in Chlorella vulgaris cultivation. Bioresour Technol 170:144–151CrossRefPubMedGoogle Scholar
  17. Ledda C, Romero Villegas GI, Adani F, Acién FG, Molina E (2015) Utilization of centrate from wastewater treatment for the outdoor production of Nannochloropsis gaditana biomass at pilot-scale. Algal Res 12:17–25CrossRefGoogle Scholar
  18. Marcilhac C, Sialve B, Pourcher AM, Ziebal C, Bernet N, Béline F (2014) Digestate color and light intensity affect nutrient removal and competition phenomena in a microalgal-bacterial ecosystem. Water Res 64:278–287CrossRefPubMedGoogle Scholar
  19. Masojídek J, Kopecký J, Giannelli L, Torzillo G (2011) Productivity correlated to photobiochemical performance of Chlorella mass cultures grown outdoors in thin-layer cascades. J Ind Microbiol Biotechnol 38:307–317CrossRefPubMedGoogle Scholar
  20. Mehrabadi A, Craggs R, Farid MM (2015) Wastewater treatment high rate algal ponds (WWT HRAP) for low-cost biofuel production. Bioresour Technol 184:202–214CrossRefPubMedGoogle Scholar
  21. Mendoza JL, Granados MR, de Godos I, Acién FG, Molina E, Banks C, Heaven S (2013) Fluid-dynamic characterization of real-scale raceway reactors for microalgae production. Biomass Bioenergy 54:267–275CrossRefGoogle Scholar
  22. Morales-Amaral MM, Gómez-Serrano C, Acién FG, Fernández-Sevilla JM, Molina-Grima E (2015a) Outdoor production of Scenedesmus sp. in thin-layer and raceway reactors using centrate from anaerobic digestion as the sole nutrient source. Algal Res 12:99–108CrossRefGoogle Scholar
  23. Morales-Amaral MM, Gómez-Serrano C, Acién FG, Fernández-Sevilla JM, Molina-Grima E (2015b) Production of microalgae using centrate from anaerobic digestion as the nutrient source. Algal Res 9:297–305CrossRefGoogle Scholar
  24. Muñoz R, Guieysse B (2006) Algal-bacterial processes for the treatment of hazardous contaminants: a review. Water Res 40:2799–2815CrossRefPubMedGoogle Scholar
  25. Muñoz R, Jacinto M, Guieysse B, Mattiasson B (2005) Combined carbon and nitrogen removal from acetonitrile using algal-bacterial bioreactors. Appl Microbiol Biotechnol 67:699–707CrossRefPubMedGoogle Scholar
  26. Olguín EJ (2012) Dual purpose microalgae-bacteria-based systems that treat wastewater and produce biodiesel and chemical products within a Biorefinery. Biotechnol Adv 30:1031–1046CrossRefPubMedGoogle Scholar
  27. Palmer CM (1969) A composite rating of algae tolerating organic pollution. J Phycol 5:78–82CrossRefPubMedGoogle Scholar
  28. Park JB, Craggs RJ (2010) Wastewater treatment and algal production in high rate algal ponds with carbon dioxide addition. Water Sci Technol 61(3):633–639CrossRefPubMedGoogle Scholar
  29. Park JBK, Craggs RJ, Shilton AN (2011) Recycling algae to improve species control and harvest efficiency from a high rate algal pond. Water Res 45:6637–6649CrossRefPubMedGoogle Scholar
  30. Passos F, Ferrer I (2015) Influence of hydrothermal pretreatment on microalgal biomass anaerobic digestion and bioenergy production. Water Res 68:364–373CrossRefPubMedGoogle Scholar
  31. Posadas E, García-Encina PA, Domínguez A, Díaz I, Becares E, Blanco S, Muñoz R (2014) Enclosed tubular and open algal-bacterial biofilm photobioreactors for carbon and nutrient removal from domestic wastewater. Ecol Eng 67:156–164CrossRefGoogle Scholar
  32. Posadas E, Morales MM, Gomez C, Acién FG, Muñoz R (2015) Influence of pH and CO2 source on the performance of microalgae-based secondary domestic wastewater treatment in outdoors pilot raceways. Chem Eng J 265:239–248CrossRefGoogle Scholar
  33. Posten C (2009) Design principles of photo-bioreactors for cultivation of microalgae. Eng Life Sci 9:165–177CrossRefGoogle Scholar
  34. Romero García JM, Acién FG, Fernández Sevilla JM (2012) Development of a process for the production of l-amino-acids concentrates from microalgae by enzymatic hydrolysis. Bioresour Technol 112:164–170CrossRefPubMedGoogle Scholar
  35. Ruiz-Marin A, Mendoza-Espinosa LG, Stephenson T (2010) Growth and nutrient removal in free and immobilized green algae in batch and semi-continuous cultures treating real wastewater. Bioresour Technol 101:58–64CrossRefPubMedGoogle Scholar
  36. Sepúlveda C, Acién FG, Gómez C, Jiménez-Ruíz N, Riquelme C, Molina-Grima E (2015) Utilization of centrate for the production of the marine microalgae Nannochloropsis gaditana. Algal Res 9:107–116CrossRefGoogle Scholar
  37. Vasseur C, Bougaran G, Garnier M, Hamelin J, Leboulanger C, Le Chevanton M, Mostajir B, Sialve B, Steyer JP, Fouilland E (2012) Carbon conversion efficiency and population dynamics of a marine algae-bacteria consortium growing on simplified synthetic digestate: first step in a bioprocess coupling algal production and anaerobic digestion. Bioresour Technol 119:79–87CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • F. Gabriel Acién
    • 1
    Email author
  • C. Gómez-Serrano
    • 1
  • M. M. Morales-Amaral
    • 1
  • J. M. Fernández-Sevilla
    • 1
  • E. Molina-Grima
    • 1
  1. 1.Department of Chemical EngineeringUniversity of AlmeríaAlmeríaSpain

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