Applied Microbiology and Biotechnology

, Volume 100, Issue 21, pp 9103–9110 | Cite as

A novel cell autolysis system for cost-competitive downstream processing

Biotechnological products and process engineering

Abstract

The industrial production of low value-added biological products poses significant challenges due to cost pressures. In recent years, it has been argued that synthetic biology approaches will lead to breakthroughs that eliminate price bottlenecks for the production of a wide range of biological products including bioplastics and biofuels. One significant bottleneck lies in the necessity to break the tough cell walls of microbes in order to release intracellular products. We here report the implementation of the first synthetic biology standard part based on the lambda phage SRRz genes and a synthetic ribosome binding site (RBS) that works in Escherichia coli and Halomonas campaniensis, which enables the producer strains to induce lysis after the addition of small amounts (1–5 %) of solvents or to spontaneously lyse during the stresses of downstream processing, and thus has the potential to eliminate the mechanical cell disruption step as both an efficiency bottleneck and a significant capex barrier when implementing downstream bioprocesses.

Keywords

Autolysis PHB PHA Bioplastics Halomonas Synthetic biology 

Supplementary material

253_2016_7669_MOESM1_ESM.pdf (928 kb)
ESM 1(PDF 928 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Peking-Tsinghua Center for Life Sciences, School of Life ScienceTsinghua UniversityBeijingChina
  2. 2.Center for Synthetic and Systems BiologyTsinghua UniversityBeijingChina
  3. 3.MOE Key Lab of Industrial BiocatalysisTsinghua UniversityBeijingChina

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