Applied Microbiology and Biotechnology

, Volume 100, Issue 18, pp 8135–8146 | Cite as

Electron transfer pathways of formate-driven H2 production in Desulfovibrio

  • Mónica Martins
  • Cláudia Mourato
  • Fabio O. Morais-Silva
  • Claudina Rodrigues-Pousada
  • Gerrit Voordouw
  • Judy D. Wall
  • Inês A. C. Pereira
Applied microbial and cell physiology
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Abstract

The potential of sulfate-reducing bacteria (SRB) as biocatalysts for H2 production from formate was recently demonstrated, but the electron transfer pathways involved were not described. In the present work, we analyzed the H2 production capacity of five Desulfovibrio strains: Desulfovibrio vulgaris, Desulfovibrio desulfuricans, Desulfovibrio alaskensis, Desulfovibrio fructosivorans, and Desulfovibrio gigas. D. vulgaris showed the highest H2 productivity (865 mL Lmedium −1), and D. gigas the lowest one (374 mL Lmedium −1 of H2). The electron transfer pathways involved in formate-driven H2 production by these two organisms were further investigated through the study of deletion mutants of hydrogenases (Hases) and formate dehydrogenases (Fdhs). In D. vulgaris, the periplasmic FdhAB is the key enzyme for formate oxidation and two pathways are apparently involved in the production of H2 from formate: a direct one only involving periplasmic enzymes and a second one that involves transmembrane electron transfer and may allow energy conservation. In the presence of selenium, the Hys [NiFeSe] Hase is the main periplasmic enzyme responsible for H2 production, and the cytoplasmic Coo Hase is apparently involved in the ability of D. vulgaris to grow by converting formate to H2, in sparging conditions. Contrary to D. vulgaris, H2 production in D. gigas occurs exclusively by the direct periplasmic route and does not involve the single cytoplasmic Hase, Ech. This is the first report of the metabolic pathways involved in formate metabolism in the absence of sulfate in SRB, revealing that the electron transfer pathways are species-specific.

Keywords

H2 production Desulfovibrio Metabolic pathway Hydrogenases Formate dehydrogenases 
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Notes

Acknowledgments

This research was supported by Post-Doc and PhD fellowships SFRH/BPD/76707/2011 and SFRH/BD/86442/2012, grants UID/Multi/04551/2013 and PTDC/BBB-EBB/2723/2014 funded by Fundação para a Ciência e Tecnologia (Portugal). The authors are grateful to Sebastien Dementin for supplying D. fructosivorans and Sofia Venceslau for genomic analysis of this organism.

Compliance with ethical standards

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Funding

This study was funded by Fundação para a Ciência e Tecnologia (Portugal) (fellowships numbers SFRH/BPD/76707/2011 and SFRH/BD/86442/2012; grants UID/Multi/04551/2013 and PTDC/BBB-EBB/2723/2014).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

253_2016_7649_MOESM1_ESM.pdf (272 kb)
ESM 1 (PDF 271 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Mónica Martins
    • 1
  • Cláudia Mourato
    • 1
  • Fabio O. Morais-Silva
    • 1
  • Claudina Rodrigues-Pousada
    • 1
  • Gerrit Voordouw
    • 2
  • Judy D. Wall
    • 3
    • 4
  • Inês A. C. Pereira
    • 1
  1. 1.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)Universidade Nova de LisboaOeirasPortugal
  2. 2.Department of Biological SciencesUniversity of CalgaryCalgaryCanada
  3. 3.Biochemistry DepartmentUniversity of MissouriColumbiaUSA
  4. 4.ENIGMA (Ecosystems and Networks Integrated with Genes and Molecular Assemblies)BerkeleyUSA

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