Microbial diversity and community structure in an antimony-rich tailings dump
- 805 Downloads
To assess the impact of antimony (Sb) on microbial community structure, 12 samples were taken from an Sb tailings pile in Guizhou Province, Southwest China. All 12 samples exhibited elevated Sb concentrations, but the mobile and bioaccessible fractions were small in comparison to total Sb concentrations. Besides the geochemical analyses, microbial communities inhabiting the tailing samples were characterized to investigate the interplay between the microorganisms and environmental factors in mine tailings. In all samples, Proteobacteria and Actinobacteria were the most dominant phyla. At the genus level, Thiobacillus, Limnobacter, Nocardioides, Lysobacter, Phormidium, and Kaistobacter demonstrated relatively high abundances. The two most abundant genera, Thiobacillus and Limnobacter, are characterized as sulfur-oxidizing bacteria and thiosulfate-oxidizing bacteria, respectively, while the genus Lysobacter contains arsenic (As)-resistant bacteria. Canonical correspondence analysis (CCA) indicates that TOC and the sulfate to sulfide ratio strongly shaped the microbial communities, suggesting the influence of the environmental factors in the indigenous microbial communities.
KeywordIllumina sequencing Antimony Sulfur-oxidizing bacteria Canonical correspondence analysis
Compliance with ethical standards
This research was funded by the Public Welfare Foundation of the Ministry of Water Resources of China (201501011), the National Natural Science Foundation of China (41103080, 41173028), the Opening Fund of the State Key Laboratory of Environmental Geochemistry (SKLEG2015907), and Guangdong Academy of Sciences (REN  20).
Conflict of interest
The authors declare that he has no conflict of interest.
This article does not contain any studies with human participants performed by any of the authors.
- Claus G, Kutzner HJ (1985) Physiology and kinetics of autotrophic denitrification by Thiobacillus denitrificans. Appl Microbiol Biotechnol 22:283–288Google Scholar
- Communities of the Europe (1976) Council directive 76/464/EEC of 4 May 1976 on pollution caused by certain dangerous substances discharged into the aquatic environment of the community.Google Scholar
- Dold B, Fontboté L (2001) Element cycling and secondary mineralogy in porphyry copper tailings as a function of climate, primary mineralogy, and mineral processing. J Geochem Explor 74:3–55Google Scholar
- Edgar RC (2013) UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nat Method 10:996–998Google Scholar
- Fornieles AC, de Torres AG, Alonso EV, Cordero MS, Pavón JC (2011) Speciation of antimony (III) and antimony (V) in seawater by flow injection solid phase extraction coupled with online hydride generation inductively coupled plasma mass spectrometry. J Anal At Spectrom 26:1619–1626CrossRefGoogle Scholar
- Fowler B, Goering P (1991) Antimony. Metals and their compounds in the environment: occurrence, analysis, and biological relevance. Weinheim, pp:743–750Google Scholar
- Glöckner FO, Zaichikov E, Belkova N, Denissova L, Pernthaler J, Pernthaler A, Amann R (2000) Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of actinobacteria. Appl Environ Microbiol 66:5053–5065CrossRefPubMedPubMedCentralGoogle Scholar
- Johnson M, Bradshaw A (1977) Prevention of heavy metal pollution from mine wastes by vegetative stabilization. Trans Inst Min Metall 86:47–55Google Scholar
- Kimbrough DE, Wakakuwa JR (2002) Acid digestion for sediments, sludges, soils, and solid wastes. a proposed alternative to EPA SW 846 Method 3050. Environ Sci Technol 25:898–900Google Scholar
- Kishimoto N, Kosako Y, Tano T (1991) Acidobacterium capsulatum gen. nov., sp. nov.: an acidophilic chemoorganotrophic bacterium containing menaquinone from acidic mineral environment. Curr Microbiol 22:1–7Google Scholar
- Lepš J, Šmilauer P (2003) Multivariate analysis of ecological data using CANOCO. Cambridge University Press, UKGoogle Scholar
- Lu H, Sato Y, Fujimura R, Nishizawa T, Kamijo T, Ohta H (2011) Limnobacter litoralis sp. nov., a thiosulfate-oxidizing, heterotrophic bacterium isolated from a volcanic deposit, and emended description of the genus Limnobacter. Int J Syst Evol Microbiol 61:404–407Google Scholar
- Luo G, Shi Z, Wang G (2012) Lysobacter arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from iron-mined soil. Int J Syst Evol Microbiol 62:1659–1665Google Scholar
- Navarro-Noya YE, Jan-Roblero J, del Carmen González-Chávez M, Hernández-Gama R, Hernández-Rodríguez C (2010) Bacterial communities associated with the rhizosphere of pioneer plants (Bahia xylopoda and Viguiera linearis) growing on heavy metals-contaminated soils. Anton Leeuw Int J G 97:335–349CrossRefGoogle Scholar
- Ritchie VJ, Ilgen AG, Mueller SH, Trainor TP, Goldfarb RJ (2013) Mobility and chemical fate of antimony and arsenic in historic mining environments of the Kantishna Hills district, Denali National Park and Preserve. Alaska Chem Geol 335:172–188. doi: 10.1016/j.chemgeo.2012.10.016 CrossRefGoogle Scholar
- Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541CrossRefPubMedPubMedCentralGoogle Scholar
- Schumacher BA (2002) Methods for the determination of total organic carbon (TOC) in soils and sediments. Ecological Risk Assessment Support Center, United State EPAGoogle Scholar
- Sekiguchi Y, Muramatsu M, Imachi H, Narihiro T, Ohashi A, Harada H, Hanada S, Kamagata Y (2008) Thermodesulfovibrio aggregans sp. nov. and Thermodesulfovibrio thiophilus sp. nov., anaerobic, thermophilic, sulfate-reducing bacteria isolated from thermophilic methanogenic sludge, and emended description of the genus Thermodesulfovibrio. Int J Syst Evol Microbiol 58:2541–2548Google Scholar
- Smith NA, Kelly DP (1988) Oxidation of carbon disulphide as the sole source of energy for the autotrophic growth of Thiobacillus thioparus strain TK-m. J Gener Microbiol 134:3041–3048Google Scholar
- Spring S, Kämpfer P, Schleifer KH (2001) Limnobacter thiooxidans gen. nov., sp. nov., a novel thiosulfate-oxidizing bacterium isolated from freshwater lake sediment. Int J Syst Evol Microbiol 51:1463–1470Google Scholar
- USEPA (1979) Water related fate of the 129 priority pollutants. USEPA, WashingtonGoogle Scholar