Characterization of biosurfactants produced by Lactobacillus spp. and their activity against oral streptococci biofilm
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Lactic acid bacteria (LAB) can interfere with pathogens through different mechanisms; one is the production of biosurfactants, a group of surface-active molecules, which inhibit the growth of potential pathogens. In the present study, biosurfactants produced by Lactobacillus reuteri DSM 17938, Lactobacillus acidophilus DDS-1, Lactobacillus rhamnosus ATCC 53103, and Lactobacillus paracasei B21060 were dialyzed (1 and 6 kDa) and characterized in term of reduction of surface tension and emulsifying activity. Then, aliquots of the different dialyzed biosurfactants were added to Streptococcus mutans ATCC 25175 and Streptococcus oralis ATCC 9811 in the culture medium during the formation of biofilm on titanium surface and the efficacy was determined by agar plate count, biomass analyses, and flow cytometry. Dialyzed biosurfactants showed abilities to reduce surface tension and to emulsifying paraffin oil. Moreover, they significantly inhibited the adhesion and biofilm formation on titanium surface of S. mutans and S. oralis in a dose-dependent way, as demonstrated by the remarkable decrease of cfu/ml values and biomass production. The antimicrobial properties observed for dialyzed biosurfactants produced by the tested lactobacilli opens future prospects for their use against microorganisms responsible of oral diseases.
KeywordsBiosurfactants Lactobacillus Chemical characterization Oral streptococci Biofilm formation percentages
This work was supported by partial grants from PIO SODALIZIO dei PICENI Foundation (Rome, Italy).
Compliance with ethical standards
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.
Conflict of interest
The authors declare that they have no conflict of interest.
- Barbesti S, Citterio S, Labra M, Baroni MD, Neri MG, Sgorbati S (2000) Two and three-color fluorescence flow cytometric analysis of immunoidentified viable bacteria. Cytometry 40:214–218. doi: 10.1002/1097-0320(20000701)40:3<214::AID-CYTO6>3.0.CO;2-M CrossRefPubMedGoogle Scholar
- Çaglar E, Cildir SK, Ergeneli S, Sandalli N, Twetman S (2006) Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta Odontol Scand 64:314–318. doi: 10.1080/00016350600801709 CrossRefPubMedGoogle Scholar
- Kuiper I, Lagendijk EL, Pickford R, Derrick JP, Lamers GEM, Thomas-Oates JE, Lugtenberg BJJ, Bloemberg GV (2004) Characterization of two Pseudomonas putida lipopeptide biosurfactants, putisolvin I and II, which inhibit biofilm formation and break down existing biofilms. Mol Microbiol 51:97–113. doi: 10.1046/j.1365-2958.2003.03751.x CrossRefPubMedGoogle Scholar
- Meurman JH, Stamatova I (2011) Lactic acid bacteria in oral health. in: lactic acid bacteria in oral health, lactic acid bacteria: microbiological and functional aspects. CRC Press, pp 403–422Google Scholar
- Saharan BS, Sahu RK, Sharma D (2012) A review on biosurfactants: fermentation, current developments and perspectives. Genet Eng Biotechnol J 1:1–14Google Scholar
- Sharma D, Saharan BS (2014) Simultaneous production of biosurfactants and bacteriocins by probiotic Lactobacillus casei MRTL3. Int J Microbiol Article ID 698713 . doi: 10.1155/2014/6987137 pages
- Sharma D, Saharan BS, Chauhan N, Bansal A, Procha S (2014) Production and structural characterization of Lactobacillus helveticus derived biosurfactant. Sci World J Article ID:493548 . doi: 10.1155/2014/4935489 pages
- Spinler JK, Taweechotipatr M, Rognerud CL, Ou CN, Tumwasorn S, Versalovic J (2008) Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe 14:166–171. doi: 10.1016/j.anaerobe.2008.02.001 CrossRefPubMedPubMedCentralGoogle Scholar