Applied Microbiology and Biotechnology

, Volume 100, Issue 13, pp 5781–5789 | Cite as

2,3-Butanediol production from cellobiose using exogenous beta-glucosidase-expressing Bacillus subtilis

  • Kosuke Tanimura
  • Shingo Takashima
  • Takuya Matsumoto
  • Tsutomu Tanaka
  • Akihiko Kondo
Biotechnological products and process engineering
First Online:
Received:
Revised:
Accepted:

Abstract

We engineered efficient 2,3-butanediol (23BD) production from cellobiose using Bacillus subtilis. First, we found that B. subtilis harboring an empty vector could produce 23BD from cellobiose. However, productivity using cellobiose as a carbon source was lower than that when using glucose. This lower productivity was improved by adding purified beta-glucosidase from Thermobifida fusca YX (Tfu_0937) in the fermentation. Encouraged by these findings, we found that hydrolysis of cellobiose to glucose was an important reaction of 23BD biosynthesis in B. subtilis using cellobiose. Hence, we created efficient 23BD production from cellobiose using exogenous Tfu_0937-expressing B. subtilis. Using the engineered strain, 21.2 g L−1 of 23BD was produced after 72 h of cultivation. The productivity and yield were 0.294 g L−1 h−1 and 0.35 g 23BD/g cellobiose, respectively. We successfully demonstrated efficient 23BD production from cellobiose by using BGL-expressing B. subtilis.

Keywords

2,3-Butanediol Cellobiose B. subtilis Beta-glucosidase 
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Notes

Acknowledgments

This work was supported by Special Coordination Funds for Promoting Science and Technology, provided by the Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan.

Compliance with ethical standards

Ethical approval

This study does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Kosuke Tanimura
    • 1
  • Shingo Takashima
    • 1
  • Takuya Matsumoto
    • 2
  • Tsutomu Tanaka
    • 1
  • Akihiko Kondo
    • 1
  1. 1.Department of Chemical Science and Engineering, Graduate School of EngineeringKobe UniversityKobeJapan
  2. 2.Organization of Advanced Science and TechnologyKobe UniversityKobeJapan

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