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Applied Microbiology and Biotechnology

, Volume 100, Issue 10, pp 4385–4393 | Cite as

Comparative characterization of physicochemical properties and bioactivities of polysaccharides from selected medicinal mushrooms

  • Chun-Han Su
  • Min-Nan Lai
  • Ching-Chuan Lin
  • Lean-Teik Ng
Biotechnological products and process engineering

Abstract

Mushroom polysaccharides have been known to possess various pharmacological activities. However, information on their chemical and biological differences between mushrooms remains limited. In this study, we aimed to examine the differences in physicochemical characteristics of polysaccharides prepared from Antrodia cinnamomea (AC-P), Coriolus versicolor (CV-P), Grifola frondosa (GF-P), Ganoderma lucidum (GL-P), and Phellinus linteus (PL-P), followed by evaluating their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Results showed that under similar conditions of preparation, the monosaccharide composition of polysaccharides varied between different mushrooms, and glucose was the predominant monosaccharide, followed by galactose and mannose. AC-P and GF-P contained the highest amount of (1,3;1,6)-β-d-glucans. The degree of branching of (1,3;1,6)-β-d-glucans in all polysaccharides ranged from 0.21 to 0.26, with the exception of GF-P (0.38). The molecular weights of different polysaccharides showed diverse distributions; AC-P, CV-P, and GF-P contained two major macromolecular populations (< 30 and >200 kDa) and possessed triple-helix conformation, whereas GL-P (10.2 kDa) and PL-P (15.5 kDa) only had a low molecular weight population without triple-helix structure. These polysaccharides showed different inhibitory potency on NO production in LPS-stimulated RAW264.7 cells.

Keywords

Polysaccharides β-Glucans Physicochemical properties Medicinal mushrooms 

Notes

Acknowledgments

The authors would like to thank the Ministry of Science and Technology of Taiwan for partial funding of this study under grant number MOST 104-2622-B-002-006-CC2.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Chun-Han Su
    • 1
  • Min-Nan Lai
    • 2
  • Ching-Chuan Lin
    • 3
  • Lean-Teik Ng
    • 1
  1. 1.Department of Agricultural ChemistryNational Taiwan UniversityTaipeiTaiwan
  2. 2.Kang Jian Biotech Co., Ltd.NantouTaiwan
  3. 3.Ten-In Food Co., Ltd.ChiayiTaiwan

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