Applied Microbiology and Biotechnology

, Volume 100, Issue 10, pp 4375–4383 | Cite as

Advanced functionalization of polyhydroxyalkanoate via the UV-initiated thiol-ene click reaction

Biotechnological products and process engineering

Abstract

Polyhydroxyalkanoates (PHAs) incorporating vinyl-bearing 3-hydroxyalkanoates were prepared in 8.5–12.9 g L−1 yield. The molar ratios (0–16 mol%) of the vinyl-bearing 3-hydroxyalkanoate derivatives were controlled by the continuous feeding of undecylenate at various concentrations. Subsequently, the PHAs were functionalized by UV-initiated thiol-ene click reaction and chemical modification. 1H NMR spectra suggested that 3-mercaptopropionic acid and 2-aminoethanethiol were successfully introduced into the vinyl-bearing PHA. Subsequently, chemical modification using fluorescein or a fibronectin active fragment (GRGDS) was attempted. The former yielded a PHA derivative capable of emitting fluorescence under UV irradiation, which was useful for determining the miscibility of PHA in a composite film comprising poly-ʟ-lactic acid (PLLA) and PHA. In the latter case, PHA bearing GRGDS peptides exhibited cell adhesiveness, suggesting that its biocompatibility was improved upon peptide introduction. Taken together, the UV-initiated thiol-ene click reaction was demonstrated to be useful in PHA modification.

Keywords

Polyhydroxyalkanoate Medium-chain length Click chemistry Double bond Ene-thiol click reaction 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Division of Applied Chemistry, Faculty of EngineeringHokkaido UniversitySapporoJapan
  2. 2.Department of Materials ChemistryAsahikawa National College of TechnologyAsahikawaJapan

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