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Applied Microbiology and Biotechnology

, Volume 100, Issue 1, pp 347–360 | Cite as

Trophic regulation of autoaggregation in Pseudomonas taiwanensis VLB120

  • Karolin Schmutzler
  • Octavia Natascha Kracht
  • Andreas Schmid
  • Katja BuehlerEmail author
Applied microbial and cell physiology

Abstract

Five mutants of Pseudomonas taiwanensis VLB120ΔCeGFP showed significant autoaggregation when growing on defined carbohydrates or gluconate, while they grew as suspended cells on complex medium and on organic acids like citrate and succinate. Surprisingly, the respective mutations affected very different genes, although all five strains exhibited the same behaviour of aggregate formation. To elucidate the mechanism of the aggregative behaviour, the microbial adhesion to hydrocarbons (MATH) assay and contact angle measurements were performed that pointed to an increased cell surface hydrophobicity. Moreover, investigations of the outer layer of the cell membrane revealed a reduced amount of O-specific polysaccharides in the lipopolysaccharide of the mutant cells. To determine the regulation of the aggregation, reverse transcription quantitative real-time PCR was performed and, irrespective of the mutation, the transcription of a gene encoding a putative phosphodiesterase, which is degrading the global second messenger cyclic diguanylate, was decreased or even deactivated in all mutants. In summary, it appears that the trophic autoaggregation was regulated via cyclic diguanylate and a link between the cellular cyclic diguanylate concentration and the lipopolysaccharide composition of P. taiwanensis VLB120ΔCeGFP is suggested.

Keywords

Autoaggregation Cyclic diguanylate Lipopolysaccharide Hydrophobicity Carbon source BifA 

Notes

Acknowledgments

We thank Sebastian Glonke of Laboratory of Thermodynamics, TU Dortmund University, for his support during contact angle measurements. We are grateful to Monika Meuris of Laboratory of Biomaterials and Polymer Sciences, TU Dortmund University, for her help and support during SEM preparation. We thank Carl Zeiss Microscopy GmbH for providing the confocal laser scanning microscope. Karolin Schmutzler was funded by a personal grant from the Ministry of Innovation, Science and Research of North Rhine-Westphalia, in the frame of the CLIB-Graduate Cluster Industrial Biotechnology, contract no. 314-108 00108.

Compliance with ethical standards

Funding

This study was funded by the Ministry of Innovation, Science and Research of North Rhine-Westphalia in the frame of CLIB-Graduate Cluster Industrial Biotechnology (contract no. 314-108 001 08).

Conflict of interest

The authors declare that they have no competing interests.

Human and animal rights and informed consent

This article does not contain any studies with human or animal subjects.

Supplementary material

253_2015_7006_MOESM1_ESM.pdf (1.9 mb)
ESM 1 (PDF 1.89 MB)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Karolin Schmutzler
    • 1
    • 2
  • Octavia Natascha Kracht
    • 1
  • Andreas Schmid
    • 2
  • Katja Buehler
    • 2
    Email author
  1. 1.Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical EngineeringTU Dortmund UniversityDortmundGermany
  2. 2.Department of Solar MaterialsHelmholtz-Centre for Environmental Research - UFZLeipzigGermany

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