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Applied Microbiology and Biotechnology

, Volume 99, Issue 23, pp 10215–10224 | Cite as

DasR is a pleiotropic regulator required for antibiotic production, pigment biosynthesis, and morphological development in Saccharopolyspora erythraea

  • Cheng-Heng Liao
  • Ya Xu
  • Sébastien Rigali
  • Bang-Ce YeEmail author
Applied microbial and cell physiology

Abstract

The GntR-family transcription regulator, DasR, was previously identified as pleiotropic, controlling the primary amino sugar N-acetylglucosamine (GlcNAc) and chitin metabolism in Saccharopolyspora erythraea and Streptomyces coelicolor. Due to the remarkable regulatory impact of DasR on antibiotic production and development in the model strain of S. coelicolor, we here identified and characterized the role of DasR to secondary metabolite production and morphological development in industrial erythromycin-producing S. erythraea. The physiological studies have shown that a constructed deletion of dasR in S. erythraea resulted in antibiotic, pigment, and aerial hyphae production deficit in a nutrient-rich condition. DNA microarray assay, combined with quantitative real-time reverse transcription PCR (qRT-PCR), confirmed these results by showing the downregulation of the genes relating to secondary metabolite production in the dasR null mutant. Notably, electrophoretic mobility shift assays (EMSA) showed DasR as being the first identified regulator that directly regulates the pigment biosynthesis rpp gene cluster. In addition, further studies indicated that GlcNAc, the major nutrient signal of DasR-responsed regulation, blocked secondary metabolite production and morphological development. The effects of GlcNAc were shown to be caused by DasR mediation. These findings demonstrated that DasR is an important pleiotropic regulator for both secondary metabolism and morphological development in S. erythraea, providing new insights for the genetic engineering of S. erythraea with increased erythromycin production.

Keywords

Saccharopolyspora erythraea DasR Secondary metabolism Morphological development 

Notes

Acknowledgments

This work was supported by grants from the National Science Foundation of China (21276079), SRFDP 20120074110009 of the Chinese Ministry of Education, the National Key Technologies R&D Programs (2014AA02150 and 22007AA02Z331), and Fundamental Research Funds for the Central Universities.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

253_2015_6892_MOESM1_ESM.pdf (47 kb)
Table S1 (PDF 47 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Cheng-Heng Liao
    • 1
  • Ya Xu
    • 1
  • Sébastien Rigali
    • 3
  • Bang-Ce Ye
    • 1
    • 2
    Email author
  1. 1.Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina
  2. 2.School of Chemistry and Chemical EngineeringShihezi UniversityXinjiangChina
  3. 3.Centre for Protein Engineering, Institut de Chimie B6aUniversity of LiègeLiègeBelgium

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