Identification and biochemical characterization of a novel endo-type β-agarase AgaW from Cohnella sp. strain LGH
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An agar-degrading bacterium, strain LGH, was isolated and identified as Cohnella sp. This strain had a capability of utilizing agar as a sole carbon source for growth and showed a strong agarolytic activity. A novel endo-type β-agarase gene agaW, encoding a primary translation product of 891 amino acids, including a 26 amino acid signal peptide, was cloned and identified from a genomic library of strain LGH. The AgaW belonged to the glycoside hydrolase (GH) GH50 family, with less than 39 % amino acid sequence similarity with any known protein, and hydrolyzed agarose into neoagarotetraose as the major end product and neoagarobiose as the minor end product through other neoagarooligosaccharide intermediates, such as neoagarohexaose.
KeywordsCohnella sp. LGH Endo-type β-agarase Neoagarotetraose Neoagarobiose
This study was funded by the Fundamental Research Funds for the Central Universities of The People’s Republic of China (Project nos. KYZ201409 and KJQN201517) and the National Natural Science Foundation (Project nos. 31470551, 41401347, and 41401254).
Conflict of interest
The authors declare that they have no competing interests.
This article does not contain any studies with human participants performed by any of the authors.
- Araki C (1959) Seaweed polysaccharides. In: Wolfrom ML (ed) Carbohydrate chemistry of substances of biological interest. Pergamon Press, London, pp. 15–30Google Scholar
- Duckworth M, Yaphe W (1972) The relationship between structures and biological properties of agars. In: Nisizawa K (ed) Proceedings of the 7th international seaweed symposium. Halstead Press, New York, pp. 15–22Google Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Weiner RM, Taylor 2nd LE, Henrissat B, Hauser L, Land M, Coutinho PM, Rancurel C, Saunders EH, Longmire AG, Zhang H, Bayer EA, Gilbert HJ, Larimer F, Zhulin IB, Ekborg NA, Lamed R, Richardson PM, Borovok I, Hutcheson S (2008) Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2–40 T. PLoS Genet 4:e1000087PubMedCentralCrossRefPubMedGoogle Scholar