Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes
- 949 Downloads
Phospholipids (PLs) containing specific polar head groups and fatty acids, artificially synthesized from a complex mixture of natural PLs, have considerable industrial applications. The biocatalytic approaches to synthesizing structured PLs are of great interest because the enzymes used show high selectivity and performance under mild conditions, leading to the generation of products that cannot easily be obtained by chemical catalysis. Although the limited supply of phospholipases (e.g., phospholipase D) has thus far been an obstacle to the widespread use of enzymatic processing, recent advances in enzyme preparation have opened up various applications for PL modification. In this review, attempts to increase the productivity and utility of microbial phospholipases and lipases are presented. We also summarize recent developments in enzyme-catalyzed modification of PLs, focusing particularly on the relevant reactions, bioreactor design, and novel proof-of-concept experiments.
KeywordsBioreactor Enzyme production Lecithin Phospholipases Phospholipid modification
This work was supported by grants-in-aid from the Cross-ministerial Strategic Innovation Promotion Program (SIP) from Japan Science and Technology Agency, Japan.
Conflict of interest
The authors declare that they have no competing interests.
- Estiasih T, Ahmadi K, Ginting E, Priyanto AD (2013) Modification of soy crude lecithin by partial enzymatic hydrolysis using phospholipase A1. Int Food Res J 20:843–849Google Scholar
- Zhao T, No DS, Kim BH, Garcia HS, Kim Y, Kim IH (2014) Immobilized phospholipase A1-catalyzed modification of phosphatidylcholine with n-3 polyunsaturated fatty acid. Food Biochem 157:132–140Google Scholar