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Applied Microbiology and Biotechnology

, Volume 99, Issue 12, pp 5363–5371 | Cite as

Improving furfural tolerance of Zymomonas mobilis by rewiring a sigma factor RpoD protein

  • Fu-Rong Tan
  • Li-Chun Dai
  • Bo Wu
  • Han Qin
  • Zong-Xia Shui
  • Jing-Li Wang
  • Qi-Li Zhu
  • Qi-Chun Hu
  • Zhi-Yong Ruan
  • Ming-Xiong HeEmail author
Bioenergy and biofuels

Abstract

Furfural from lignocellulosic hydrolysates is the key inhibitor for bio-ethanol fermentation. In this study, we report a strategy of improving the furfural tolerance in Zymomonas mobilis on the transcriptional level by engineering its global transcription sigma factor (σ70, RpoD) protein. Three furfural tolerance RpoD mutants (ZM4-MF1, ZM4-MF2, and ZM4-MF3) were identified from error-prone PCR libraries. The best furfural-tolerance strain ZM4-MF2 reached to the maximal cell density (OD600) about 2.0 after approximately 30 h, while control strain ZM4-rpoD reached its highest cell density of about 1.3 under the same conditions. ZM4-MF2 also consumed glucose faster and yield higher ethanol; expression levels and key Entner-Doudoroff (ED) pathway enzymatic activities were also compared to control strain under furfural stress condition. Our results suggest that global transcription machinery engineering could potentially be used to improve stress tolerance and ethanol production in Z. mobilis.

Keywords

Furfural tolerance Ethanol production Global transcription machinery engineering Error-prone PCR Sigma factor RpoD protein 

Notes

Acknowledgments

This work was supported by Applied Basic Research Programs of Sichuan province (NO. 2014JY0065) and partially supported by Youth Science and Technology Foundation of Sichuan Province in China (Grant NO: 2015JQ0047). Open Funds of Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin (Tarim University, BRZD1403). Open Funds of State Key Laboratory of Microbial Technology (Shandong University, M2013-07), Open Funds of Key Laboratory of Microbial Resources Collection and Preservation (Ministry of Agriculture, MOA, 2013),

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Fu-Rong Tan
    • 1
  • Li-Chun Dai
    • 1
  • Bo Wu
    • 1
  • Han Qin
    • 1
  • Zong-Xia Shui
    • 1
  • Jing-Li Wang
    • 1
  • Qi-Li Zhu
    • 1
  • Qi-Chun Hu
    • 1
    • 2
  • Zhi-Yong Ruan
    • 3
  • Ming-Xiong He
    • 1
    • 2
    Email author
  1. 1.Biogas Institute of Ministry of AgricultureBiomass Energy Technology Research CentreChengduPeople’s Republic of China
  2. 2.Key Laboratory of Development and Application of Rural Renewable EnergyMinistry of AgricultureChengduPeople’s Republic of China
  3. 3.Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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