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Applied Microbiology and Biotechnology

, Volume 99, Issue 16, pp 6813–6829 | Cite as

Evidence for cholesterol-lowering activity by Bifidobacterium bifidum PRL2010 through gut microbiota modulation

  • Ilaria Zanotti
  • Francesca TurroniEmail author
  • Antonio Piemontese
  • Leonardo Mancabelli
  • Christian Milani
  • Alice Viappiani
  • Gilda Prevedini
  • Borja Sanchez
  • Abelardo Margolles
  • Lisa Elviri
  • Bernini Franco
  • Douwe van Sinderen
  • Marco Ventura
Applied microbial and cell physiology

Abstract

Bifidobacteria are members of the human gut microbiota, which are known to influence the metabolic abilities of their host. Here, we investigated the capabilities of bifidobacteria to reduce cholesterol levels in synthetic growth media, clearly demonstrating assimilation of this molecule by particular bifidobacterial strains, including Bifidobacterium bifidum PRL2010 (LMG S-28692). The transcriptomic analysis of PRL2010 cells cultivated in the presence of cholesterol revealed a significantly increased transcription level of genes encoding putative transporters and reductases, indicative of specific mechanisms for cholesterol assimilation as well as cholesterol conversion to coprostanol. Cholesterol lowering activity of B. bifidum PRL2010 cells was further evaluated by means of an in vivo murine model, showing that the fecal microbiota of mice is modified toward those bacteria involved in the metabolism of cholesterol.

Keywords

Bifidobacterium Cholesterol Gut microbiota Functional genomics 

Notes

Acknowledgments

We thank GenProbio srl for financial support of the Laboratory of Probiogenomics. This work was financially supported by a FEMS Jensen Award to FT. FT is a members of The Alimentary Pharmabiotic Centre, while DvS is also a member of the Alimentary Glycoscience Research Cluster, both funded by Science Foundation Ireland (SFI), through the Irish Government’s National Development Plan (Grant numbers SFI/12/RC/2273 and 08/SRC/B1393, respectively).

Conflict of interest

The authors declare no financial or commercial conflict of interest.

Compliance with the ethical standards

The authors declare that all the procedures used for murine experiments (housing and experiments) were conducted in accordance with the animal experimentation European Communities Council Directive of 24 November 1986 (86/EEC) and approved by the ethical commission of the University of Parma and by the Italian Institute of Health.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Ilaria Zanotti
    • 1
  • Francesca Turroni
    • 2
    Email author
  • Antonio Piemontese
    • 1
  • Leonardo Mancabelli
    • 3
  • Christian Milani
    • 3
  • Alice Viappiani
    • 4
  • Gilda Prevedini
    • 3
  • Borja Sanchez
    • 5
    • 6
  • Abelardo Margolles
    • 5
  • Lisa Elviri
    • 1
  • Bernini Franco
    • 1
  • Douwe van Sinderen
    • 2
  • Marco Ventura
    • 3
  1. 1.Department of PharmacyUniversity of ParmaParmaItaly
  2. 2.Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience InstituteNational University of IrelandCorkIreland
  3. 3.Laboratory of Probiogenomics, Department of Life SciencesUniversity of ParmaParmaItaly
  4. 4.GenProbio srlParmaItaly
  5. 5.Dairy Research Institute of AsturiasSpanish National Research Council (IPLA-CSIC)VillaviciosaSpain
  6. 6.Department of Analytical Chemistry and Food Science, Faculty of Food Science and TechnologyUniversity of VigoVigoSpain

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