Separation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer
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A new molecularly imprinted polymer (MIP), prepared by hispidin as the template molecule, was synthesized and applied as an adsorbent phase for solid phase extraction (SPE) to isolate and enrich hispidin from eight species of mushrooms. The optimization of synthesis and the adsorption behaviors of the MIPs were investigated in detail. In comparison with C18-SPE, MIP-SPE displayed high selectivity and good affinity for hispidin for extract of Phellinus igniarius. The antioxidant activity of the extracts after using the MIPs was evaluated by free radical scavenging activity, and inhibition of erythrocyte hemolysis, and lipid peroxidation. This developed method provided a rapid, selective, and effective approach for separation and enrichment of active compounds from the natural products.
KeywordsHispidin Molecularly imprinted polymer Mushrooms Antioxidant activity
We thank the support of this research by ‘863’ Program of China (grant no. 2012AA022105D), Tianjin Natural Science Foundation (grant no. 10JCYBJC09800), and National Natural Science Foundation.
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