Applied Microbiology and Biotechnology

, Volume 99, Issue 18, pp 7569–7577 | Cite as

Separation and purification of the antioxidant compound hispidin from mushrooms by molecularly imprinted polymer

  • Ning Li
  • Lijie Zhao
  • Tzi Bun NgEmail author
  • Jack Ho Wong
  • Yan Yan
  • Zhong Shi
  • Fang LiuEmail author
Biotechnologically relevant enzymes and proteins


A new molecularly imprinted polymer (MIP), prepared by hispidin as the template molecule, was synthesized and applied as an adsorbent phase for solid phase extraction (SPE) to isolate and enrich hispidin from eight species of mushrooms. The optimization of synthesis and the adsorption behaviors of the MIPs were investigated in detail. In comparison with C18-SPE, MIP-SPE displayed high selectivity and good affinity for hispidin for extract of Phellinus igniarius. The antioxidant activity of the extracts after using the MIPs was evaluated by free radical scavenging activity, and inhibition of erythrocyte hemolysis, and lipid peroxidation. This developed method provided a rapid, selective, and effective approach for separation and enrichment of active compounds from the natural products.


Hispidin Molecularly imprinted polymer Mushrooms Antioxidant activity 



We thank the support of this research by ‘863’ Program of China (grant no. 2012AA022105D), Tianjin Natural Science Foundation (grant no. 10JCYBJC09800), and National Natural Science Foundation.

Supplementary material

253_2015_6499_MOESM1_ESM.pdf (209 kb)
ESM 1 (PDF 208 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjinChina
  2. 2.Key Laboratory of Original Environmental Quality of MOAAgro-Environmental Protection Institute of Ministry of AgricultureTianjinPeople’s Republic of China
  3. 3.Tianjin Key Laboratory of Agro-Environment and Agro-Product SafetyTianjinPeople’s Republic of China
  4. 4.School of Biomedical Sciences, Faculty of MedicineThe Chinese University of Hong Kong, ShatinHong KongChina
  5. 5.Department of Microbiology, College of Life ScienceNankai UniversityTianjinChina

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