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Applied Microbiology and Biotechnology

, Volume 99, Issue 10, pp 4343–4353 | Cite as

Relationships between the genome and some phenotypical properties of Lactobacillus fermentum CECT 5716, a probiotic strain isolated from human milk

  • Nivia Cárdenas
  • Jonathan E. Laiño
  • Susana Delgado
  • Esther Jiménez
  • Marianela Juárez del Valle
  • Graciela Savoy de Giori
  • Fernando Sesma
  • Baltasar Mayo
  • Leónides Fernández
  • Jean Guy LeBlanc
  • Juan M. RodríguezEmail author
Applied genetics and molecular biotechnology

Abstract

Lactobacillus fermentum CECT 5716, isolated from human milk, has immunomodulatory, anti-inflammatory, and anti-infectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain. In this work, some phenotypic properties of L. fermentum CECT 5716 were evaluated, and the genetic basis for the obtained results was searched for in the strain genome. L. fermentum CECT 5716 does not contain plasmids and showed neither bacteriocin nor biogenic amine biosynthesis ability but was able to produce organic acids, glutathione, riboflavin, and folates and to moderately stimulate the maturation of mouse dendritic cells. No prophages could be induced, and the strain was sensitive to all antibiotics proposed by European Food Safety Authority (EFSA) standards, while no transmissible genes potentially involved in antibiotic resistance were detected in its genome. Globally, there was an agreement between the phenotype properties of L. fermentum CECT 5716 and the genetic information contained in its genome.

Keywords

Lactobacillus fermentum Genome Glutathione Riboflavin Folate Antibiotic resistance Dendritic cells Human milk 

Notes

Acknowledgments

This work was supported by CSD2007-00063 (FUN-C-FOOD, Consolider-Ingenio 2010), CYTED-IBEROFUN, and AGL2013-41980-P projects from the Ministerio de Ciencia e Innovación (Spain), a research contract funded by Biosearch Life (Granada, Spain) and by funding from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Argentina.

Supplementary material

253_2015_6429_MOESM1_ESM.pdf (422 kb)
ESM 1 (PDF 421 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Nivia Cárdenas
    • 1
    • 2
  • Jonathan E. Laiño
    • 3
  • Susana Delgado
    • 4
  • Esther Jiménez
    • 1
    • 2
  • Marianela Juárez del Valle
    • 3
  • Graciela Savoy de Giori
    • 3
  • Fernando Sesma
    • 3
  • Baltasar Mayo
    • 4
  • Leónides Fernández
    • 1
    • 2
  • Jean Guy LeBlanc
    • 3
  • Juan M. Rodríguez
    • 1
    • 2
    Email author
  1. 1.Departamento de Nutrición, Bromatología y Tecnología de los AlimentosUniversidad Complutense de MadridMadridSpain
  2. 2.ProbisearchMadridSpain
  3. 3.CERELA-CONICET, Centro de Referencia para LactobacilosSan Miguel de TucumánArgentina
  4. 4.Instituto de Productos Lácteos de Asturias (IPLA-CSIC)VillaviciosaSpain

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