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Applied Microbiology and Biotechnology

, Volume 99, Issue 13, pp 5683–5696 | Cite as

Biostimulation of petroleum-hydrocarbon-contaminated marine sediment with co-substrate: involved metabolic process and microbial community

  • Zhen Zhang
  • Irene M. C. LoEmail author
Environmental biotechnology

Abstract

This study investigated the effect of acetate and methanol as co-substrates on anaerobic biodegradation of total petroleum hydrocarbons (TPHs, C10–C40) in marine sediment. The findings evidenced that the degradation of TPH can be enhanced by adding acetate or methanol. The addition of acetate was generally more favorable than the addition of methanol for the TPH degradation. Both sulfate reduction and methanogenesis occurred in the acetate-treated sediment. However, the depletion of SO4 2− inhibited sulfate reduction over the incubation period. Only methanogenesis was prevalent in the methanol-treated sediment within the whole incubation period. The degradation of TPH fractions with higher carbon number ranges (C31–C40) was speculated to be more favored under sulfate-reducing condition, while TPH fractions with lower carbon number ranges (C10–C20) were preferentially degraded under methanogenic condition. The 16S rRNA clone library–based analysis revealed that the addition of different co-substrates led to distinct structures of the microbial community. Clones related to sulfate-reducing Desulfobacterales were the most abundant in the sediment dosed with acetate. Clones related to Clostridiales predominated in the sediment dosed with methanol. Acetoclastic methanogens were found to be the predominant archaeal species in the sediment dosed with acetate, while both acetoclastic methanogens and hydrogenotrophic methanogens accounted for large proportions in the sediment dosed with methanol. The results obtained in this study will contribute to more comprehensive knowledge on the role of acetate and methanol as co-substrates in biostimulation of petroleum-hydrocarbon-contaminated marine sediment.

Keywords

Biostimulation Co-substrate Metabolic process Microbial community Total petroleum hydrocarbons (TPHs) 

Notes

Acknowledgments

The authors wish to thank the General Research Fund of Hong Kong Research Grants Council for providing financial support for this research study. We also thank Dr. Ming-fei Shao (Assistant Professor of Harbin Institute of Technology Shenzhen Graduate School) for his help in molecular identification and analysis of microbial community structure in sediment.

Supplementary material

253_2015_6420_MOESM1_ESM.pdf (307 kb)
ESM 1 (PDF 307 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Civil and Environmental EngineeringThe Hong Kong University of Science and TechnologyHong KongChina

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