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Applied Microbiology and Biotechnology

, Volume 99, Issue 5, pp 2267–2275 | Cite as

Development of a novel uric-acid-responsive regulatory system in Escherichia coli

  • Chaoning Liang
  • Dandan Xiong
  • Yi Zhang
  • Shanshan Mu
  • Shuang-Yan Tang
Applied genetics and molecular biotechnology

Abstract

A novel uric-acid-responsive regulatory system was developed in Escherichia coli by adapting the HucR-related regulatory elements from Deinococcus radiodurans into E. coli. The induction performance of this system was compared to the performance of both the pBAD and pET systems. Our novel regulatory system was induced in a dose-dependent manner in the presence of uric acid and exhibited low basal expression in its absence. The system was characterized by a wide dynamic range of induction, being compatible with various E. coli strains and not requiring genomic modifications of the bacterial host. E. coli DH5α and DH10B were the most suitable host strains for optimal performance of this system. In conclusion, we developed a regulatory system with potential for applications in both recombinant protein expression and metabolic optimization.

Keywords

Regulatory protein Uric acid Dynamic range Induction fold Escherichia coli 

Notes

Acknowledgments

The authors would like to thank Mr. Ye Mao for his technical assistance in this research. This work was supported by the Ministry of Science and Technology of China Grant 2013CB734003, the National Natural Science Foundation of China Grant 21172095, and the Key Research Program of the Chinese Academy of Sciences Grant KSZD-EW-Z-015-2.

Supplementary material

253_2014_6290_MOESM1_ESM.pdf (88 kb)
ESM 1 (PDF 88 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Chaoning Liang
    • 1
  • Dandan Xiong
    • 1
    • 2
  • Yi Zhang
    • 1
    • 2
  • Shanshan Mu
    • 1
    • 2
  • Shuang-Yan Tang
    • 1
  1. 1.CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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