Effect of sulfate addition on methane production and sulfate reduction in a mesophilic acetate-fed anaerobic reactor
- 507 Downloads
A mesophilic anaerobic moving bed biofilm reactor (MBBR) was operated to evaluate the effect of sulfate addition on methane production and sulfate reduction using acetate as the sole carbon source. The results show that at the organic loading rate of 4.0 g TOC/L/day, the TOC removal efficiencies and the biogas production rates achieved over 95 % and 7000 mL/L/day without sulfate, respectively, and slightly decreased with sulfate addition (500–800 mg/L). Methane production capacities were not influenced significantly with the addition of sulfate, while sulfate reduction efficiencies were not stable with 23–87 % in the acetate-fed reactor. Fluorescent in situ hybridization (FISH) was used to analyze the functional microbial compositions of acetate-degrading methane-producing bacteria (MPB) and sulfate-reducing bacteria (SRB) in the reactor. The results found that as the increase of sulfate concentration, the proportion of Methanomicrobiales increased up to 58 ± 2 %, while Methanosaeta and Methanosarcina decreased. The dominant methanogens shifted into hydrogenotrophic methanogens from even distribution of acetoclastic and hydrogenotrophic methanogens. When hydrogenotrophic methanogens were dominant, sulfate reduction efficiency was high, while sulfate reduction efficiency was low as acetoclastic methanogens were dominant.
KeywordsAcetate degradation Anaerobic digestion Methane production Sulfate reduction
This work was supported by National Nature Science Foundation of China (No. 51208324) and Ministry of Education of China (New Century Distinguished Young Scientist Supporting Plan, No. NCET-13-0387).
- Amann RI (1995) In situ identification of micro-organisms by whole-cell hybridization with rRNA-targeted nucleic acid probes. In: Kowalchuk GA, de Bruijn FJ, Head IM, Akkermans ADL, van Elsas JD (eds) Molecular microbial ecology manual, 2nd edn. Kluwer Academic Publishers, the Netherlands, pp 1–15Google Scholar
- APHA (1999) Standard methods for the examination of water and wastewater. American Public Health Association, WashingtonGoogle Scholar
- Elferink S, Visser A, Pol LWH, Stams AJM (1994) Sulfate reduction in methanogenic bioreactors. FEMS Microbiol Rev 15:119–136Google Scholar
- Parkin GF, Lynch NA, Kuo WC, Van Keuren EL, Bhattacharya SK (1990) Interaction between sulfate reducers and methanogens fed acetate and propionate. J Water Pollut Control Fed 62:780–788Google Scholar