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Applied Microbiology and Biotechnology

, Volume 99, Issue 3, pp 1427–1433 | Cite as

Promiscuous activity of (S,S)-butanediol dehydrogenase is responsible for glycerol production from 1,3-dihydroxyacetone in Corynebacterium glutamicum under oxygen-deprived conditions

  • Toru Jojima
  • Takafumi Igari
  • Yasuhiro Moteki
  • Masako Suda
  • Hideaki Yukawa
  • Masayuki Inui
Applied microbial and cell physiology

Abstract

Corynebacterium glutamicum can consume glucose to excrete glycerol under oxygen deprivation. Although glycerol synthesis from 1,3-dihydroxyacetone (DHA) has been speculated, no direct evidence has yet been provided in C. glutamicum. Enzymatic and genetic investigations here indicate that the glycerol is largely produced from DHA and, unexpectedly, the reaction is catalyzed by (S,S)-butanediol dehydrogenase (ButA) that inherently catalyzes the interconversion between S-acetoin and (S,S)-2,3-butanediol. Consequently, the following pathway for glycerol biosynthesis in the bacterium emerges: dihydroxyacetone phosphate is dephosphorylated by HdpA to DHA, which is subsequently reduced to glycerol by ButA. This study emphasizes the importance of promiscuous activity of the enzyme in vivo.

Keywords

Corynebacterium glutamicum Glycerol 2,3-Butanediol dehydrogenase ButA Enzyme promiscuity 

Notes

Acknowledgments

We thank Crispinus A. Omumasaba (RITE) for critical reading of the manuscript.

Supplementary material

253_2014_6170_MOESM1_ESM.pdf (178 kb)
ESM 1 (PDF 178 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Toru Jojima
    • 1
  • Takafumi Igari
    • 1
  • Yasuhiro Moteki
    • 1
  • Masako Suda
    • 1
  • Hideaki Yukawa
    • 1
  • Masayuki Inui
    • 1
    • 2
  1. 1.Research Institute of Innovative Technology for the EarthKizugawaJapan
  2. 2.Graduate School of Biological SciencesNara Institute of Science and TechnologyIkomaJapan

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