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Applied Microbiology and Biotechnology

, Volume 99, Issue 4, pp 1947–1955 | Cite as

Biofilm bacterial communities in urban drinking water distribution systems transporting waters with different purification strategies

  • Huiting Wu
  • Jingxu Zhang
  • Zilong Mi
  • Shuguang XieEmail author
  • Chao ChenEmail author
  • Xiaojian Zhang
Environmental biotechnology

Abstract

Biofilm formation in drinking water distribution systems (DWDS) has many adverse consequences. Knowledge of microbial community structure of DWDS biofilm can aid in the design of an effective control strategy. However, biofilm bacterial community in real DWDS and the impact of drinking water purification strategy remain unclear. The present study investigated the composition and diversity of biofilm bacterial community in real DWDSs transporting waters with different purification strategies (conventional treatment and integrated treatment). High-throughput Illumina MiSeq sequencing analysis illustrated a large shift in the diversity and structure of biofilm bacterial community in real DWDS. Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Nitrospirae, and Cyanobacteria were the major components of biofilm bacterial community. Proteobacteria (mainly Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria) predominated in each DWDS biofilm, but the compositions of the dominant proteobacterial classes and genera and their proportions varied among biofilm samples. Drinking water purification strategy could shape DWDS biofilm bacterial community. Moreover, Pearson’s correlation analysis indicated that Actinobacteria was positively correlated with the levels of total alkalinity and dissolved organic carbon in tap water, while Firmicutes had a significant positive correlation with nitrite nitrogen.

Keywords

Biofilm Distribution system Microbial community Illumina sequencing Pipe Proteobacteria 

Notes

Acknowledgments

This work was financially supported by the State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (No. MARC2012D010), National Water Special Program (No. 2012ZX07404-002), and International Science and Technology Cooperation Program of China (No. 2010DFA91830).

Supplementary material

253_2014_6095_MOESM1_ESM.pdf (164 kb)
ESM 1 (PDF 164 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC)Tsinghua UniversityBeijingChina
  2. 2.School of EnvironmentTsinghua UniversityBeijingChina
  3. 3.State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and EngineeringPeking UniversityBeijingChina

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