Applied Microbiology and Biotechnology

, Volume 98, Issue 22, pp 9217–9228 | Cite as

Fed-batch strategies using butyrate for high cell density cultivation of Pseudomonas putida and its use as a biocatalyst

  • Federico Cerrone
  • Gearoid Duane
  • Eoin Casey
  • Reeta Davis
  • Ian Belton
  • Shane T. Kenny
  • Maciej W. Guzik
  • Trevor Woods
  • Ramesh P. Babu
  • Kevin O’ConnorEmail author
Biotechnological products and process engineering


A mathematically based fed-batch bioprocess demonstrated the suitability of using a relatively cheap and renewable substrate (butyric acid) for Pseudomonas putida CA-3 high cell density cultivation. Butyric acid fine-tuned addition is critical to extend the fermentation run and avoid oxygen consumption while maximising the biomass volumetric productivity. A conservative submaximal growth rate (μ of 0.25 h−1) achieved 71.3 g L−1 of biomass after 42 h of fed-batch growth. When a more ambitious feed rate was supplied in order to match a μ of 0.35 h−1, the volumetric productivity was increased to 2.0 g L−1 h−1, corresponding to a run of 25 h and 50 g L−1 of biomass. Both results represent the highest biomass and the best biomass volumetric productivity with butyrate as a sole carbon source. However, medium chain length polyhydroxyalkanoate (mcl-PHA) accumulation with butyrate grown cells is low (4 %). To achieve a higher mcl-PHA volumetric productivity, decanoate was supplied to butyrate grown cells. This strategy resulted in a PHA volumetric productivity of 4.57 g L−1 h−1 in the PHA production phase and 1.63 g L−1 h−1over the lifetime of the fermentation, with a maximum mcl-PHA accumulation of 65 % of the cell dry weight.


High cell density Fed-batch bioprocess Butyric acid Biocatalyst Polyhydroxyalkanoate 



Federico Cerrone was funded by Enterprise Ireland and the Irish Industrial Development Agency (IDA) through the Technology Center for Biorefining and Bioenergy (project no. CC20090004). The authors have no conflict of interest to declare.

Supplementary material

253_2014_5989_MOESM1_ESM.pdf (144 kb)
ESM 1 (PDF 143 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Federico Cerrone
    • 1
    • 2
  • Gearoid Duane
    • 3
  • Eoin Casey
    • 3
  • Reeta Davis
    • 1
    • 2
  • Ian Belton
    • 1
  • Shane T. Kenny
    • 1
  • Maciej W. Guzik
    • 1
  • Trevor Woods
    • 4
  • Ramesh P. Babu
    • 2
    • 4
    • 5
  • Kevin O’Connor
    • 1
    • 2
    • 6
    • 7
    Email author
  1. 1.School of Biomolecular and Biomedical ScienceUniversity College DublinDublin 4Ireland
  2. 2.Technology Centre for Biorefining and BioenergyDublinIreland
  3. 3.School of Biochemical and Bioprocessing EngineeringUniversity College DublinDublin 4Ireland
  4. 4.School of PhysicsTrinity College DublinDublin 2Ireland
  5. 5.Centre for Research on Adaptive Nanostructure and NanodevicesTrinity College DublinDublin 2Ireland
  6. 6.Earth InstituteUniversity College DublinDublinIreland
  7. 7.The UCD Conway InstituteDublinIreland

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