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Applied Microbiology and Biotechnology

, Volume 98, Issue 17, pp 7645–7657 | Cite as

Identification of oleaginous yeast strains able to accumulate high intracellular lipids when cultivated in alkaline pretreated corn stover

  • Irnayuli R. Sitepu
  • Mingjie Jin
  • J. Enrique Fernandez
  • Leonardo da Costa Sousa
  • Venkatesh Balan
  • Kyria L. Boundy-Mills
Bioenergy and biofuels

Abstract

Microbial oil is a potential alternative to food/plant-derived biodiesel fuel. Our previous screening studies identified a wide range of oleaginous yeast species, using a defined laboratory medium known to stimulate lipid accumulation. In this study, the ability of these yeasts to grow and accumulate lipids was further investigated in synthetic hydrolysate (SynH) and authentic ammonia fiber expansion (AFEX™)-pretreated corn stover hydrolysate (ACSH). Most yeast strains tested were able to accumulate lipids in SynH, but only a few were able to grow and accumulate lipids in ACSH medium. Cryptococcus humicola UCDFST 10-1004 was able to accumulate as high as 15.5 g/L lipids, out of a total of 36 g/L cellular biomass when grown in ACSH, with a cellular lipid content of 40 % of cell dry weight. This lipid production is among the highest reported values for oleaginous yeasts grown in authentic hydrolysate. Preculturing in SynH media with xylose as sole carbon source enabled yeasts to assimilate both glucose and xylose more efficiently in the subsequent hydrolysate medium. This study demonstrates that ACSH is a suitable medium for certain oleaginous yeasts to convert lignocellullosic sugars to triacylglycerols for production of biodiesel and other valuable oleochemicals.

Keywords

Lignocellulosic Cryptococcus humicola Biodiesel Energy Oleochemics 

Notes

Acknowledgments

Some yeasts used in this study isolated and identified as part of a collaborative project with the Government of the Republic of Indonesia, funded by Grant Number U01TW008160 from the NIH Fogarty International Center, the NIH Office of Dietary Supplements, the National Science Foundation and the Department of Energy. This project was supported by the USDA Agricultural Food Research Initiative of the National Food and Agriculture, USDA, Grant #35621-04750. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Fogarty International Center or the National Institutes of Health, the Office of Dietary Supplements, the National Science Foundation, the Department of Energy, or the Department of Agriculture. Atit Kanti and Agustinus Joko Nugroho of LIPI Biology, Indonesia, and Sarah Asih Faulina of FORDA, Ministry of Forestry, Indonesia, were thanked for the help with isolation of Indonesia microbes. Erin Cathcart and Silviana Tjahyono of UC Davis are thanked for their technical assistance. The authors thanked the anonymous reviewers for providing valuable feedback that significantly improved the quality of the manuscript.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Irnayuli R. Sitepu
    • 1
    • 2
  • Mingjie Jin
    • 3
  • J. Enrique Fernandez
    • 1
  • Leonardo da Costa Sousa
    • 3
  • Venkatesh Balan
    • 3
  • Kyria L. Boundy-Mills
    • 1
  1. 1.Phaff Yeast Culture Collection, Department of Food Science and TechnologyUniversity of CaliforniaDavisUSA
  2. 2.Forestry Research and Development Agency (FORDA)The Ministry of ForestryBogorIndonesia
  3. 3.Biomass Conversion Research Laboratory, Department of Chemical Engineering and Material ScienceMichigan State UniversityEast LansingUSA

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