Applied Microbiology and Biotechnology

, Volume 98, Issue 20, pp 8685–8695 | Cite as

Effects of antimicrobial peptide L-K6, a temporin-1CEb analog on oral pathogen growth, Streptococcus mutans biofilm formation, and anti-inflammatory activity

  • Dejing Shang
  • Hao Liang
  • Shi Wei
  • Xin Yan
  • Qingzu Yang
  • Yue Sun
Applied microbial and cell physiology

Abstract

Dental caries and periodontitis are common bacterial mouth infections. As a potentially attractive substitute for conventional antibiotics, antimicrobial peptides have been widely tested and used for controlling bacterial infections. In this study, we tested the efficacy of the peptides from the skin secretions of Rana chensinensis for killing several major cariogenic and periodontic pathogens as well as Candida albicans. L-K6, a temporin-1CEb analog, exhibited high antimicrobial activity against the tested oral pathogens and was able to inhibit Streptococcus mutans biofilm formation and reduce 1-day-old S. mutans biofilms with a minimum biofilm inhibitory concentration and reducing concentration of 3.13 and 6.25 μM, respectively. The results of confocal laser scanning microscopy demonstrated that the peptide significantly reduced cell viability within oral biofilms. Furthermore, as little as 5 μM L-K6 significantly inhibited lipopolysaccharide (LPS)- and interleukin-1β-induced productions of interleukin-8 and tumor necrosis factor-α from THP-1 monocytic cells. This anti-inflammatory activity is associated with the binding of L-K6 to LPS and neutralizing LPS-induced proinflammatory responses in THP-1 cells, as well as dissociating LPS aggregates. Our results suggest that L-K6 may have potential clinical applications in treating dental caries by killing S. mutans within dental plaque and acting as anti-inflammatory agents in infected tissues.

Keywords

Amphibian Antimicrobial peptide Biofilm Inflammation Dental caries Lipopolysaccharide 

Supplementary material

253_2014_5927_MOESM1_ESM.pdf (716 kb)
ESM 1(PDF 716 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Dejing Shang
    • 1
    • 2
  • Hao Liang
    • 1
  • Shi Wei
    • 1
  • Xin Yan
    • 1
  • Qingzu Yang
    • 1
  • Yue Sun
    • 1
  1. 1.Liaoning Provincial Key Laboratory of Biotechnology and Drug DiscoveryLiaoning Normal UniversityDalianPeople’s Republic of China
  2. 2.Faculty of Life ScienceLiaoning Normal UniversityDalianPeople’s Republic of China

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