Applied Microbiology and Biotechnology

, Volume 98, Issue 17, pp 7447–7456 | Cite as

Production of 5,8-dihydroxy-9,12(Z,Z)-octadecadienoic acid from linoleic acid by whole recombinant Escherichia coli cells expressing diol synthase from Aspergillus nidulans

  • Min-Ju Seo
  • Kyung-Chul Shin
  • Deok-Kun OhEmail author
Biotechnologically relevant enzymes and proteins


Diol synthase from Aspergillus nidulans was cloned and expressed in Escherichia coli. Recombinant E. coli cells expressing diol synthase from A. nidulans converted linoleic acid to a product that was identified as 5,8-dihydroxy-9,12(Z,Z)-octadecadienoic acid by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The recombinant cells and the purified enzyme showed the highest activity for linoleic acid among the fatty acids tested. The optimal reaction conditions for the production of 5,8-dihydroxy-9,12(Z,Z)-octadecadienoic acid from linoleic acid using whole recombinant E. coli cells expressing diol synthase were pH 7.5, 35°C, 250 rpm, 5 g l−1 linoleic acid, 23 g l−1 cells, and 20% (v/v) dimethyl sulfoxide in a 250-ml baffled flask. Under these optimized conditions, whole recombinant cells expressing diol synthase produced 4.98 g l−1 5,8-dihydroxy-9,12(Z,Z)-octadecadienoic acid for 150 min without detectable byproducts, with a conversion yield of 99% (w/w) and a productivity of 2.5 g l−1 h−1. This is the first report on the biotechnological production of dihydroxy fatty acid using whole recombinant cells expressing diol synthase.


Aspergillus nidulans Linoleic acid 5,8-Dihydroxy-9,12(Z,Z)-octadecadienoic acid production Diol synthase Recombinant Escherichia coli 



This study was supported by grants from the Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries (No. 112002-3) and the Korea Healthcare Technology R&D Project, Ministry for Health & Welfare, Republic of Korea (No. 2012-009).

Supplementary material

253_2014_5709_MOESM1_ESM.pdf (282 kb)
ESM 1 (PDF 281 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Bioscience and BiotechnologyKonkuk UniversitySeoulSouth Korea

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