Kinetic and stoichiometric characterization of anoxic sulfide oxidation by SO-NR mixed cultures from anoxic biotrickling filters
Monitoring the biological activity in biotrickling filters is difficult since it implies estimating biomass concentration and its growth yield, which can hardly be measured in immobilized biomass systems. In this study, the characterization of a sulfide-oxidizing nitrate-reducing biomass obtained from an anoxic biotrickling filter was performed through the application of respirometric and titrimetric techniques. Previously, the biomass was maintained in a continuous stirred tank reactor under steady-state conditions resulting in a growth yield of 0.328 ± 0.045 g VSS/g S. To properly assess biological activity in respirometric tests, abiotic assays were conducted to characterize the stripping of CO2 and sulfide. The global mass transfer coefficient for both processes was estimated. Subsequently, different respirometric tests were performed: (1) to solve the stoichiometry related to the autotrophic denitrification of sulfide using either nitrate or nitrite as electron acceptors, (2) to evaluate the inhibition caused by nitrite and sulfide on sulfide oxidation, and (3) to propose, calibrate, and validate a kinetic model considering both electron acceptors in the overall anoxic biodesulfurization process. The kinetic model considered a Haldane-type equation to describe sulfide and nitrite inhibitions, a non-competitive inhibition to reflect the effect of sulfide on the elemental sulfur oxidation besides single-step denitrification since no nitrite was produced during the biological assays.
KeywordsHydrogen sulfide Biotrickling filter SO-NR culture Kinetics and stoichiometry Respirometry Titrimetry
The Spanish government provided financial support through the CICYT project CTM2009-14338-C03 and CTM2012-37927-C03. The Department of Chemical Engineering at UAB (Universitat Autònoma de Barcelona) is a unit of Biochemical Engineering of the Xarxa de Referència en Biotecnologia de Catalunya (XRB), Generalitat de Catalunya. The authors are grateful to Martin Ramirez, Antonio Valle, and Fernando Almengló for their great contribution to this work.
- Almenglo F, Ramírez M, Gómez JM, Cantero D (2013) H2S removal from biogás by a pilot anoxic biotrickling filter. A comparison between cocurrent and countercurrent flow operation mode increasing the loading rate. In: 5th IWA Specialized Conference on Odors and Air Emissions Jointly Held With 10th Conference on Biofiltration for Air Pollution Control, San Francisco, California, USAGoogle Scholar
- APHA-AWWA-WPCF (2005) Standard methods for the examination of water and wastewater. American Publication Health Association, WashingtonGoogle Scholar
- Artiga P, Gonzalez F, Mosquera-Corral A, Campos JL, Garrido JM, Ficara E, Mendez R (2005) Multiple analysis reprogrammable titration analyser for the kinetic characterization of nitrifying and autotrophic denitrifying biomass. Biochem Eng J 26:176–183. doi: 10.1016/j.bej.2005.04.017 CrossRefGoogle Scholar
- Gonzalez-Sanchez A, Tomas M, Dorado AD, Gamisans X, Guisasola A, Lafuente J, Gabriel D (2009) Development of a kinetic model for elemental sulfur and sulfate formation from the autotrophic sulfide oxidation using respirometric techniques. Water Sci Technol 59:1323–1329. doi: 10.2166/Wst.2009.110 PubMedCrossRefGoogle Scholar
- Heijnen JJ (2002) Bioenergetics of microbial growth encyclopedia of bioprocess technology. Wiley, New YorkGoogle Scholar
- Kristensen HG, Jorgensen PE, Henze M (1992) Characterization of functional microorganism groups and substrate in activated sludge and wastewater by AUR, NUR and OUR. Water Sci Technol 25:43–57Google Scholar
- López LR, Mora M, Gamisans X, Gabriel D (2013) Application of respirometry and titrimetry under anoxic conditions for the characterization of the carbon dioxide stripping and biological activity of a SO-NR consortium. In: 5th IWA Specialized Conference on Odors and Air Emissions Jointly Held With 10th Conference on Biofiltration for Air Pollution Control, San Francisco, California, USAGoogle Scholar
- Mora M, Guisasola A, Gamisans X, Gabriel D (2014) Examining thiosulfate-driven autotrophic denitrification through respirometry. Chemosphere (in press)Google Scholar
- Roels JA (1983) Energetics and kinetics in biotechnology. Elsevier, AmsterdamGoogle Scholar
- Syed M, Soreanu G, Falletta P, Béland M (2006) Removal of hydrogen sulfide from gas streams using biological processes—a review. Can Biosyst Eng 48:2.1–2.14Google Scholar
- Young JC, Cowan RM (2004) Respirometry for environmental science and engineering. SJ Enterprises, SpringdaleGoogle Scholar