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Applied Microbiology and Biotechnology

, Volume 98, Issue 12, pp 5417–5425 | Cite as

Effect of sodium butyrate on the assembly, charge variants, and galactosylation of antibody produced in recombinant Chinese hamster ovary cells

  • Jong Kwang Hong
  • Sang Min Lee
  • Kyung-Yong Kim
  • Gyun Min Lee
Biotechnological products and process engineering

Abstract

Sodium butyrate (NaBu) is known to increase the specific productivity of recombinant Chinese hamster ovary (rCHO) cells. To understand the effects of NaBu on the product quality, rCHO cells producing monoclonal antibody (Mab) were cultivated at various concentrations of NaBu (0 to 4 mM). NaBu increased correctly assembled Mab. In the absence of NaBu, the proportions of intact Mab (2H2L) and heavy chain dimer (2H) were 81 and 15 %. At 1 mM NaBu, the proportion of 2H2L increased to 93 %, whereas the proportion of 2H decreased to 2 %. No further increase in the proportion of 2H2L was obtained at a higher NaBu concentration. NaBu also affected the charge heterogeneity of Mab, which may affect the efficacy of Mab. The basic charge variants of Mabs increased with an increase in the NaBu concentration. In addition, NaBu affected the galactosylation of Mab negatively. Overall, the data obtained here show that NaBu used in rCHO cell cultures for improved Mab production affects certain quality aspects of Mab, in this case, the charge heterogeneity and galactosylation.

Keywords

Antibody Assembly Charge heterogeneity CHO cells Galactosylation Sodium butyrate 

Notes

Acknowledgments

This research was supported in part by the Converging Research Center Program through the NRF funded by the MEST (2009–0082276) and a grant from the Fundamental R&D Program for Technology of World Premier Materials funded by the Ministry of Knowledge Economy, Republic of Korea.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Jong Kwang Hong
    • 1
  • Sang Min Lee
    • 1
  • Kyung-Yong Kim
    • 2
  • Gyun Min Lee
    • 1
  1. 1.Department of Biological SciencesKAISTDaejeonSouth Korea
  2. 2.ISU ABXISGlobal R&D CenterSeongnamSouth Korea

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