Applied Microbiology and Biotechnology

, Volume 98, Issue 7, pp 3049–3058 | Cite as

A novel third chromosomal locus controls susceptibility to Autographa californica multiple nucleopolyhedrovirus in the silkworm, Bombyx mori

  • Jian Xu
  • Takahiro Kusakabe
  • Kimiko Yamamoto
  • Yoshitaka Suetsugu
  • Hiroaki Mon
  • Zhiqing Li
  • Li Zhu
  • Kazuhiro Iiyama
  • Yutaka Banno
  • Kaito Yoshimura
  • Jae Man LeeEmail author
Applied genetics and molecular biotechnology


Baculovirus demonstrates specific infection spectrums and thus one certain host exhibits particular response to single baculovirus isolate. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is considered to be not an innate pathogen to Bombyx mori, but some silkworm strains have been identified to be permissive to AcMNPV, indicating the positive or negative involvement of certain host factors in baculovirus replications in vivo. To provide a fundamental knowledge of this process, we performed large-scale screening to investigate the responses of 448 silkworm strains against recombinant AcMNPV inoculation. By genetic analysis between permissive and resistant strains identified, we further confirmed that a potential corresponding locus on chromosome 3 regulates host responses to AcMNPV in silkworm. Additionally, we found that it is available for AcMNPV–silkworm baculovirus expression vector system to produce proteins of interest.


Silkworm Baculovirus AcMNPV Replication Genetic analysis 



We thank Dr. Aoki (Kyushu University Graduate School) for providing the Bme21 cell line. This work was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN).

Supplementary material

253_2013_5437_MOESM1_ESM.pdf (322 kb)
ESM 1 (PDF 322 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jian Xu
    • 1
  • Takahiro Kusakabe
    • 1
  • Kimiko Yamamoto
    • 2
  • Yoshitaka Suetsugu
    • 2
  • Hiroaki Mon
    • 1
  • Zhiqing Li
    • 1
  • Li Zhu
    • 1
  • Kazuhiro Iiyama
    • 3
  • Yutaka Banno
    • 4
  • Kaito Yoshimura
    • 1
  • Jae Man Lee
    • 1
    Email author
  1. 1.Laboratory of Silkworm ScienceKyushu University Graduate School of Bioresource and Bioenvironmental SciencesFukuokaJapan
  2. 2.Insect Genome Research Unit, Agrogenomics Research CenterNational Institute of Agrobiological SciencesTsukubaJapan
  3. 3.Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate SchoolKyushu UniversityHigashi-kuJapan
  4. 4.Laboratory of Silkworm Genetic Resources, Institute of Genetic Resources, Graduate School of Bio Resources and Bioenvironmental ScienceKyushu UniversityHigashi-kuJapan

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