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Applied Microbiology and Biotechnology

, Volume 98, Issue 7, pp 3181–3190 | Cite as

Biodegradation of ofloxacin, norfloxacin, and ciprofloxacin as single and mixed substrates by Labrys portucalensis F11

  • Catarina L. Amorim
  • Irina S. Moreira
  • Alexandra S. Maia
  • Maria E. Tiritan
  • Paula M. L. CastroEmail author
Environmental biotechnology

Abstract

Fluoroquinolone (FQ) antibiotics are extensively used both in human and veterinary medicine, and their accumulation in the environment is causing an increasing concern. In this study, the biodegradation of the three most worldwide used FQs, namely ofloxacin, norfloxacin, and ciprofloxacin, by the fluoroorganic-degrading strain Labrys portucalensis F11 was assessed. Degradation occurred when the FQs were supplied individually or as mixture in the culture medium, in the presence of an easily degradable carbon source. Consumption of individual FQs was achieved at different extents depending on its initial concentration, ranging from 0.8 to 30 μM. For the lowest concentration, total uptake of each FQ was observed but stoichiometric fluoride release was not achieved. Intermediate compounds were detected and identified by LC-MS/MS with a quadrupole time of flight detector analyzer. Biotransformation of FQs by L. portucalensis mainly occurred through a cleavage of the piperazine ring and displacement of the fluorine substituent allowing the formation of intermediates with less antibacterial potency. FQ-degrading microorganisms could be useful for application in bioaugmentation processes towards more efficient removal of contaminants in wastewater treatment plants.

Keywords

Fluoroquinolones Labrys portucalensis F11 Biodegradation Metabolites 

Notes

Acknowledgments

C.L. Amorim, I.S. Moreira, and A.S. Maia wish to acknowledge a research grant from Fundação para a Ciência e Tecnologia (FCT), Portugal (ref. SFRH/BD/47109/2008, SFRH/BPD/87251/2012, and SFRH/BD/86939/2012, respectively) and Fundo Social Europeu (Programa Operacional Potencial Humano, Quadro de Referência Estratégico Nacional). This work was supported by FCT through the projects PTDC/EBB-EBI/111699/2009, CEQUIMED-Pest-OE/SAU/UI4040/2011, and PEst-OE/EQB/LA0016/2011.

Supplementary material

253_2013_5333_MOESM1_ESM.pdf (92 kb)
ESM 1 (PDF 92 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Catarina L. Amorim
    • 1
    • 2
  • Irina S. Moreira
    • 1
  • Alexandra S. Maia
    • 1
    • 3
  • Maria E. Tiritan
    • 2
    • 3
  • Paula M. L. Castro
    • 1
    Email author
  1. 1.CBQF—Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de BiotecnologiaCentro Regional do Porto da Universidade Católica Portuguesa/PortoPortoPortugal
  2. 2.CEQUIMED-UP, Laboratório de Química Orgânica e Farmacêutica, Departamento Ciências QuímicasFaculdade de Farmácia da Universidade do PortoPortoPortugal
  3. 3.Instituto Superior de Ciências da Saúde-Norte (ISCS-N)Cooperativa do Ensino Superior Politécnico e Universitário (CESPU), Centro de Investigação em Ciências da Saúde (CICS)GandraPortugal

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