Applied Microbiology and Biotechnology

, Volume 97, Issue 16, pp 7141–7150 | Cite as

Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic

  • Bo Song
  • Yan-Jun Rong
  • Ming-Xin Zhao
  • Zhen-Ming ChiEmail author
Biotechnological products and process engineering


The bacterium Bacillus amyloliquefaciens anti-CA isolated from mangrove system was found to be able to actively kill Candida albicans isolated from clinic. The bacterial strain anti-CA could produce high level of bioactive substance, amylase and protease in the cheap medium containing 2.0 % soybean meal, 2.0 % wheat flour, pH 6.5 within 26 h. After purification, the main bioactive substance was confirmed to be a cyclic lipopeptide containing a heptapeptide, L-Asp→L-Leu→L-Leu→L-Val→L-Val→L-Glu→L-Leu and a 3-OH fatty acid (15 carbons). In addition to C. albicans, the purified lipopeptide can also kill many yeast strains including Metschnikowia bicuspidata, Candida tropicalis, Yarrowia lipolytica and Saccharomyces cerevisiae. After treated by the purified lipopeptide, both the whole cells and protoplasts of C. albicans were destroyed.


B. amyloliquefaciens C. albicans Fungal pathogen Lipopeptide Surfactin 



This work was supported by grant 30771645 from National Natural Science Foundation of China.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Bo Song
    • 1
  • Yan-Jun Rong
    • 1
  • Ming-Xin Zhao
    • 1
  • Zhen-Ming Chi
    • 1
    Email author
  1. 1.Unesco Chinese Center of Marine BiotechnologyOcean University of ChinaQingdaoChina

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