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Applied Microbiology and Biotechnology

, Volume 97, Issue 15, pp 6715–6723 | Cite as

Enhanced 2,3-butanediol production in fed-batch cultures of free and immobilized Bacillus licheniformis DSM 8785

  • Ileana-Maria Jurchescu
  • Jan Hamann
  • Xiye Zhou
  • Tim Ortmann
  • Anja Kuenz
  • Ulf Prüße
  • Siegmund Lang
Biotechnological products and process engineering

Abstract

2,3-Butanediol (2,3-BD) is a valuable bulk chemical with particular use in industry. 2,3-BD has a potential as solvent and fuel additive, as carrier for pharmaceuticals, or as feedstock for the production of synthetic rubber. Until now, the highest 2,3-BD concentrations were obtained with risk group 2 microorganisms (e.g., Klebsiella oxytoca). In this study, the nonpathogenic bacterium Bacillus licheniformis DSM 8785 was used for 2,3-BD production from glucose. In batch experiments, a maximum 2,3-BD concentration of 72.6 g/L was reached from 180 g/L glucose after 86 h. The yield was 0.42 g/g glucose and the productivity was 0.86 g/(L h). During fed-batch cultivation, 2,3-BD production could be increased up to 144.7 g/L, with a productivity of 1.14 g/(L h). Additionally, repeated batch/fed-batch experiments were conducted using immobilized B. licheniformis in the form of LentiKats®. Results showed a high activity and stability of the immobilizates even after multiple medium replacements, as well as 2,3-BD concentrations, yields, and productivities similar to those obtained with free cells. To our knowledge, these results show the highest 2,3-BD concentration reported so far using a risk group 1 microorganism in general and B. licheniformis in particular. Furthermore, productivity lies in the same range with data reported from risk group 2 strains, which makes B. licheniformis DSM 8785 a suitable candidate for large-scale fermentation processes.

Keywords

2,3-Butanediol Glucose Bacillus licheniformis Fed-batch Immobilized cells LentiKats® 

Notes

Acknowledgments

This work was carried out in the framework of the European Research Area Network for Industrial Biotechnology (ERA-IB) and was funded by the Fachagentur Nachwachsende Rohstoffe e.V. (FNR) (grant no. 22009608A-E).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ileana-Maria Jurchescu
    • 1
  • Jan Hamann
    • 1
  • Xiye Zhou
    • 1
  • Tim Ortmann
    • 1
  • Anja Kuenz
    • 2
  • Ulf Prüße
    • 2
  • Siegmund Lang
    • 1
  1. 1.Department of Biotechnology, Institute for Biochemistry, Biotechnology and BioinformaticsBraunschweig University of TechnologyBraunschweigGermany
  2. 2.Institute of Agricultural TechnologyJohann Heinrich von Thünen InstituteBraunschweigGermany

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