Applied Microbiology and Biotechnology

, Volume 97, Issue 17, pp 7805–7819 | Cite as

Generation of biologically contained, readily transformable, and genetically manageable mutants of the biotechnologically important Bacillus pumilus

  • Stephanie Wemhoff
  • Friedhelm MeinhardtEmail author
Applied genetics and molecular biotechnology


Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair, as ΔuvrBA rendered them UV hypersensitive and, thus, biologically contained; they are deficient for the uracil phosphoribosyl-transferase (Δupp), allowing for 5-fluorouracil-based counterselection facilitating rapid allelic exchanges; and they are readily transformable due to the deletion of the restrictase encoding locus (ΔhsdR) of a type I restriction modification system. Vegetative growth as well as extracellular enzyme production and secretion are in no case affected. The combination of such gene deletions allows for development of B. pumilus strains suited for industrial use and further improvements.


Bacillus pumilus Extracellular enzymes Safety strain Strain improvement Transformation 



We acknowledge the skilful technical assistance of Julia Tietz. This work was supported by the Federal Ministry of Education and Research (BMBF, Bonn-Bad Godesberg, Germany), grant no. 0315594C.

Supplementary material

253_2013_4935_MOESM1_ESM.pdf (114 kb)
MOESM 1 (PDF 114 kb)


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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Institut für Molekulare Mikrobiologie und BiotechnologieWestfälische Wilhelms-Universität MünsterMünsterGermany

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