Applied Microbiology and Biotechnology

, Volume 97, Issue 12, pp 5177–5188 | Cite as

Flavoprotein oxidases: classification and applications

  • Willem P. Dijkman
  • Gonzalo de Gonzalo
  • Andrea Mattevi
  • Marco W. FraaijeEmail author


This review provides an overview of oxidases that utilise a flavin cofactor for catalysis. This class of oxidative flavoenzymes has shown to harbour a large number of biotechnologically interesting enzymes. Applications range from their use as biocatalysts for the synthesis of pharmaceutical compounds to the integration in biosensors. Through the recent developments in genome sequencing, the number of newly discovered oxidases is steadily growing. Recent progress in the field of flavoprotein oxidase discovery and the obtained biochemical knowledge on these enzymes are reviewed. Except for a structure-based classification of known flavoprotein oxidases, also their potential in recent biotechnological applications is discussed.


Flavoproteins Oxidases Biocatalysis Biosensors Flavin Oxygen 



M.W. Fraaije and A. Mattevi received support from the European Union's Seventh Framework Programme (FP7/2007-2013), project Oxygreen (grant agreement no. 212281).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Willem P. Dijkman
    • 1
  • Gonzalo de Gonzalo
    • 1
  • Andrea Mattevi
    • 2
  • Marco W. Fraaije
    • 1
    Email author
  1. 1.Molecular Enzymology Group, Groningen Biomolecular Sciences and Biotechnology InstituteUniversity of GroningenGroningenThe Netherlands
  2. 2.Department of Biology and BiotechnologyUniversity of PaviaPaviaItaly

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