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Applied Microbiology and Biotechnology

, Volume 97, Issue 13, pp 5771–5777 | Cite as

Substrate-limited co-culture for efficient production of propionic acid from flour hydrolysate

  • W. Sabra
  • D. Dietz
  • A. P. Zeng
Biotechnological products and process engineering

Abstract

Propionic acid is presently mainly produced by chemical synthesis. For many applications, especially in feed and food industries, a fermentative production of propionic acid from cheap and renewable resources is of large interest. In this work, we investigated the use of a co-culture to convert household flour to propionic acid. Batch and fed-batch fermentations of hydrolyzed flour and a process of simultaneous saccharification and fermentation were examined and compared. Fed-batch culture with substrate limitation was found to be the most efficient process, reaching a propionic acid concentration of 30 g/L and a productivity of 0.33 g/L*h. This is the highest productivity so far achieved with free cells on media containing flour hydrolysate or glucose as carbon source. Batch culture and culture with controlled saccharification and fermentation delivered significantly lower propionic acid production (17–20 g/L) due to inhibition by the intermediate product lactate. It is concluded that co-culture fermentation of flour hydrolysate can be considered as an appealing bioprocess for the production of propionic acid.

Keywords

Mixed culture Fed batch Substrate inhibition Simultaneous saccharifcation and fermentation Co-culture 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Institute of Bioprocess and Biosystems EngineeringHamburg University of TechnologyHamburgGermany
  2. 2.Microbiology Department, Faculty of ScienceAlexandria UniversityAlexandriaEgypt

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