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Applied Microbiology and Biotechnology

, Volume 97, Issue 14, pp 6189–6199 | Cite as

Metabolic flux analysis of Gluconacetobacter xylinus for bacterial cellulose production

  • Cheng Zhong
  • Gui-Cai Zhang
  • Miao Liu
  • Xin-Tong Zheng
  • Pei-Pei Han
  • Shi-Ru JiaEmail author
Biotechnological products and process engineering

Abstract

Metabolic flux analysis was used to reveal the metabolic distributions in Gluconacetobacter xylinus (CGMCC no. 2955) cultured on different carbon sources. Compared with other sources, glucose, fructose, and glycerol could achieve much higher bacterial cellulose (BC) yields from G. xylinus (CGMCC no. 2955). The glycerol led to the highest BC production with a metabolic yield of 14.7 g/mol C, which was approximately 1.69-fold and 2.38-fold greater than that produced using fructose and glucose medium, respectively. The highest BC productivity from G. xylinus CGMCC 2955 was 5.97 g BC/L (dry weight) when using glycerol as the sole carbon source. Metabolic flux analysis for the central carbon metabolism revealed that about 47.96 % of glycerol was transformed into BC, while only 19.05 % of glucose and 24.78 % of fructose were transformed into BC. Instead, when glucose was used as the sole carbon source, 40.03 % of glucose was turned into the by-product gluconic acid. Compared with BC from glucose and fructose, BC from the glycerol medium showed the highest tensile strength at 83.5 MPa, with thinner fibers and lower porosity. As a main byproduct of biodiesel production, glycerol holds great potential to produce BC with superior mechanical and microstructural characteristics.

Keywords

Bacterial cellulose Metabolic flux analysis Productivity Microstructure Crystallinity index 

Notes

Acknowledgments

The authors are grateful for the financial support from the National Natural Science Foundation of China (project no. 21106105, project no. 20976133), the Foundation of Tianjin Educational Committee (no. 20100602), and Changjiang Scholars and Innovative Research Team in University (no. IRT1166). We also gratefully acknowledge Rebecca G. Ong for her assistance in editing this manuscript.

Supplementary material

253_2013_4908_MOESM1_ESM.pdf (47 kb)
ESM 1 (PDF 46 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Cheng Zhong
    • 1
    • 2
    • 3
  • Gui-Cai Zhang
    • 1
    • 2
  • Miao Liu
    • 1
    • 2
  • Xin-Tong Zheng
    • 1
    • 2
  • Pei-Pei Han
    • 1
    • 2
  • Shi-Ru Jia
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education)Tianjin University of Science and TechnologyTianjinPeople’s Republic of China
  2. 2.School of BiotechnologyTianjin University of Science and TechnologyTianjinPeople’s Republic of China
  3. 3.Key Laboratory of Systems Bioengineering, Ministry of EducationTianjin UniversityTianjinPeople’s Republic of China

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