Applied Microbiology and Biotechnology

, Volume 97, Issue 16, pp 7297–7306 | Cite as

Rhamnolipids are conserved biosurfactants molecules: implications for their biotechnological potential

  • Amedea Perfumo
  • Michelle Rudden
  • Thomas J. P. Smyth
  • Roger Marchant
  • Paul S. Stevenson
  • Neil J. Parry
  • Ibrahim M. BanatEmail author
Applied genetics and molecular biotechnology


A range of isolates of Pseudomonas aeruginosa from widely different environmental sources were examined for their ability to synthesise rhamnolipid biosurfactants. No significant differences in the quantity or composition of the rhamnolipid congeners could be produced by manipulating the growth conditions. Sequences for the rhamnolipid genes indicated low levels of strain variation, and the majority of polymorphisms did lead to amino acid sequence changes that had no evident phenotypic effect. Expression of the rhlB and rhlC rhamnosyltransferase genes showed a fixed sequential expression pattern during growth, and no significant up-regulation could be induced by varying producer strains or growth media. The results indicated that rhamnolipids are highly conserved molecules and that their gene expression has a rather stringent control. This leaves little opportunity to manipulate and greatly increase the yield of rhamnolipids from strains of P. aeruginosa for biotechnological applications.


Rhamnolipids Biosurfactants Pseudomonas aeruginosa Bioreactor Comparative gene analysis Gene expression 



We thank Prof James Dooley at the University of Ulster for providing the clinical strains used in this study. A.P. is grateful to Dr. Urs Ochsner for his valuable advice at the beginning of this study. This work was supported by Unilever and the Department of Trade and Industry technology programme and a CAST award from the Department of Education and Learning Northern Ireland and Unilever to Michelle Rudden.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Amedea Perfumo
    • 1
  • Michelle Rudden
    • 1
  • Thomas J. P. Smyth
    • 1
  • Roger Marchant
    • 1
  • Paul S. Stevenson
    • 2
  • Neil J. Parry
    • 2
  • Ibrahim M. Banat
    • 1
    Email author
  1. 1.School of Biomedical SciencesUniversity of UlsterColeraineUK
  2. 2.Research and DevelopmentUnileverLiverpoolUK

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