Applied Microbiology and Biotechnology

, Volume 97, Issue 11, pp 4701–4712 | Cite as

Genome engineering in actinomycetes using site-specific recombinases



The rational modification of the actinomycetes genomes has a variety of applications in research, medicine, and biotechnology. The use of site-specific recombinases allows generation of multiple mutations, large DNA deletions, integrations, and inversions and may lead to significant progress in all of these fields. Despite their huge potential, site-specific recombinase-based technologies have primarily been used for simple marker removal from a chromosome. In this review, we summarise the site-specific recombination approaches for genome engineering in various actinomycetes.


Site-specific recombinases Actinomycetes Genome engineering Heterologous expression 



The work in the laboratory of AL was supported by the BMBF (GenBioCom), DFG (Lu1524/2-1) and ERC (EXPLOGEN) grants.


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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Helmholtz-Institute for Pharmaceutical Research SaarlandSaarbrückenGermany

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